Seahorse FluxPaks
Seahorse XF cell culture plates have been developed specifically to maximize the sensitivity of the sensor cartridge measurements, and provide valuable functional metabolic data from living cells, isolated mitochondria, islets, and spheroids. XF Pro M cell culture plates are now available for use with XF Pro analyzer to minimize assay edge effect and improve intra-plate consistency.
Every lot is 100% quality controlled for performance and lot-to-lot consistency.
- XF Sensor Cartridges & Cell Culture Microplates
Product Details
- Available in a wide range of configurations to accommodate 2D monolayers, 3D spheroids, suspension cells, islets, and isolated mitochondria
- Solid-state sensor probes containing polymer embedded fluorophores detect oxygen and proton levels in cell culture media
- Patented integrated injection ports for adding compounds, stimulators, inhibitors, substrates, and beads conjugated with antigens
- Patented transient microchamber provides increased analytical sensitivity and signal to noise of OCR and ECAR (PER, gPER) readings when used in an XF Analyzer
- Sensors are spotted onto the cartridge using a multi-step process that includes manual and machine vision inspection
- Conforms to ANSI/SLAS Microplate Standards for footprint dimensions and well positions
- Pre-coated Poly-D-Lysine cell culture plates, available in 96-well and 8-well formats, are available for use with XF assays using suspension cell, providing convenience and consistent assay result
- PDL cell culture plates are manufactured in an aseptic facility
- XF Pro M cell culture plates are now available for use with XF Pro analyzer to minimize assay edge effect and improve intra-plate consistency
- Designed specifically for use with Seahorse XF Analyzers
Automatic measurement of energy metabolism in real time
Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. Seahorse XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time.
Learn More- Key Literature
-
Agilent Seahorse XF Tissue Culture Microplates Specifications
Dimensional Drawings for Agilent Seahorse XF Tissue Culture Microplates
- Schematic
- English
- 09 Feb 2021
- 1.18 MB
Agilent Seahorse XF Plastic Ware Brochure
Brochure which highlights the technology behind conducting Seahorse measurements. Plate dimensions and ordering information are also included.
- Brochures
- English
- 15 Mar 2024
- 9.37 MB
- Application Notes
- Brochures
- Flyers
- Ordering Guides
- Selection Guides
- Technical Overviews
- White Papers
- FAQs
-
Will coating the wells of the microplates affect XF Assay performance?
Will coating the wells of the microplates affect XF Assay performance?
- User Manuals
Didn’t find what you need? Contact our dedicated Cell Analysis support teams.
Agilent Cell Analysis Publication Database
Search scientific publications by research area, cell type and more.
Seahorse XF Assay Learning Center
Up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.
Dr. Christoph Wilhem
Professor, University of Bonn
What we found working out on performing the assay is that now we can get away with a much lower number of cells. Now we are able to really look at the metabolic control of these rare cell types with the Seahorse Analyzer… The big advan
View MoreAgilent Cell Analysis Webinar Series
View informative Agilent Cell Analysis Technology webinars on a variety of research subjects.
The Agilent Seahorse XF Pro platform
Transforming Bioenergetics - Again. Featuring new pharma-oriented workflow solutions with improved precision, along with advanced experimental design and analysis tools, you can optimize your workflow from start to finish.
- 17 Jan 2022