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QuikChange Primer Design
QuikChange Primer Design
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The QuikChange® Primer Design Program supports mutagenic primer design for your QuikChange mutagenesis experiments. Using primer design guidelines described in QuikChange manuals, this program calculates/designs the appropriate primer sequences with the optimal melting temperature. Read
Help
for more information about the program.
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Introduction
SDM Primer Design
Principles of Minimizing Energy Cost
General Guidance
Single and Multiple Mutations
Mutagenesis of Untranslated DNA Sequence
Base Substitutions
Deletions
Insertions
Amino Acid Substitutions in Translated DNA
Genetic Code Table
Amino Acid substitution and Codon Selection
DISCLAIMER
1. It is recommended that you clear this form prior to loading each new sequence:
Clear Input
2. Select QuikChange® mutagenesis kit that you are using:
QuikChange® II
QuikChange® II XL
QuikChange®
QuikChange® XL
QuikChange® Multi
QuikChange® Lightning
Help in choosing a mutagenesis kit
3. Find your DNA sequence by pressing
Load a file from your hard-drive:
Or, paste plain text or FASTA-formatted DNA sequence in the box below:
4. Load it.
Upload Now
- or -
Upload Translated
or specify a DNA region to translate from
to
Upload Translated Region
Finally, obtain your designed primer sequences.
Design Primers
Clear this form to load a new sequence:
Clear Input
