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Analysis of Enzymatic Activity

Enzymes are highly effective in catalyzing diverse chemical reactions in a regulated, selective, and specific manner. Nearly all metabolic processes in the cell need enzyme catalysis to occur at rates fast enough to sustain life. The average cell is estimated to have approximately 1,300 different enzyme types present—all of which, apart from catalytic RNA, are proteins.

Beyond cellular metabolism studies and associated therapeutics development, purified enzymes are used in a myriad of biochemical applications to support nearly all aspects of life science research, including food production and bioremediation. 

To support these applications, Agilent offers a diverse suite of instrumentation optimized to measure enzyme activities within a broad range of formats, combining sensitive and reliable detection methods with efficient and flexible workflows. 

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Kinetic Analysis of β-Galactosidase Activity

Determination of basic enzyme kinetic parameters of β-galactosidase is described using the colorimetric substrate ONPG in microplates.

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Enzymatic Digestion of Polysaccharides, Part I

Ethanol production from grains or cellulosic material using different enzymes to hydrolyze polymers to monomeric fermentable sugars is discussed.

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Enzymatic Digestion of Polysaccharides, Part II

Learn about the optimization process for several different enzyme reactions necessary to produce fermentable sugars for bio-ethanol production.

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Studying the Impact of Temperature on Chemical Reactions

The Agilent Cary 3500 Multicell UV-Vis spectrophotometer quickly performs absorbance spectral scans on samples using multiple temperatures.

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Detection of Histone Methyltransferase and Demethylase Activity

The Synergy Neo2 is used to make automated, time-resolved fluorescence determinations to measure methylation and demethylation of histones.

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Protein Kinase Activity Monitored by Next-Generation Sensors

The BioTek Synergy Neo2 in conjunction with PhosphoSens substrates quantifies kinase activity using a direct, homogeneous, kinetic format.

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Protein Dehydrogenase Activity by Absorbance Spectroscopy

Here we describe the enzyme kinetics of alcohol dehydrogenase using the change in absorbance at 340 nm resulting from the reduction of NAD+ to NADH.

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Dynamic Monitoring of Receptor Tyrosine Kinase Activation

This application note highlights the development of an alternative receptor tyrosine kinase assay using the impedance based xCELLigence system.

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A Study of SIRT1- and SIRT2-Mediated Deacetylation

The RapidFire high-throughput MS is used to study sirtuin enzymes for histone deacetylase activity without the need for labeled substrates.

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