BioTek Synergy Neo2 Hybrid Multimode Reader
BioTek Synergy Neo2 Hybrid Multimode Reader
This HTS multimode reader features advanced environmental controls to support live-cell assays, including CO2/O2 control, incubation to 70 °C, and variable shaking. The available BioStack Neo microplate stacker delivers walk-away automation for up to 50 microplates per stack. Barcode-labeled filter cubes streamline workflows and limit errors. Powerful Gen6 software provides complete reader control, extensive data analysis, and LIMS integration.
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Product Details
- Flexible capabilities allow a wide range of screening laboratories, pharmaceutical, biotechnology, and academic facilities to achieve fast, high-performance multimode microplate detection
- With its proprietary hybrid monochromator and filter optics, Synergy Neo2 delivers both flexibility and performance for many applications
- Variable bandwidth settings enable increased sensitivity and lower limits of detection or increased specificity when multiple signals are present, reducing signal cross-talk
- Dual PMTs take two measurements simultaneously and deliver faster results than conventional systems. The fast PMT detection is ideal for FP, FRET, TR-FRET, BRET, and other ratiometric assays
- Two lasers provide increased sensitivity and fast reading speeds: a 100-mW laser for Alpha assays and a 337 nm laser for TRF assays
- Fast, automated processing of microplates using the dedicated stacker with dual plate carrier to save significant time
- Environmental controls and robust shaking profiles enable consistent data in live-cell assay workflows, including temperature control to 70 °C and CO2/O2 control
- Microvolume DNA, RNA, and protein quantification is available with the Take3 and Take3 Trio microvolume plate accessory
Automation Compatibility |
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Environment Control |
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Filter Detection Modes |
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Instrument Control |
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Monochromator Detection Modes |
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Shaking |
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Supported Labware |
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Hybrid plate reader: Flexibility and performance
With a patented combination of monochromator and filter optics, Synergy Neo2 an advanced plate reader delivering flexibility and performance for any microplate assay. Monochromator: variable bandwidth, absorbance, fluorescence, luminescence Filters: fluorescence polarization, time-resolved fluorescence, Alpha laser.
Learn MoreVariable bandwidth for sensitivity and specificity
The plate reader optics of Synergy Neo2 uses a quad monochromator design with variable bandwidth. The bandwidth can be set from 3 to 50 nm in 1 nm increment. Large bandwidth settings deliver increased sensitivity and lower limits of detection. Small bandwidth brings increased specificity when multiple signals are present.
Learn MoreTime-resolved fluorescence
Time-resolved fluorescence (TRF) measurement is very similar to fluorescence intensity (FI) measurement. The only difference is the timing of the excitation / measurement process.
Learn MoreAlpha detection
AlphaScreen is a bead-based assay technology used to study biomolecular interactions in a microplate format. The acronym 'Alpha' stands for amplified luminescent proximity homogeneous assay. This platform provides a nonradioactive, homogeneous assay that has low background and high signal to background ratios.
Learn MoreAbsorbance
Absorbance refers to the ability of a substance to absorb light of a specific wavelength. In a microplate reader designed to measure absorbance, a light source illuminates the sample using a specific wavelength, selected by an optical filter or a monochromator.
Learn MoreFluorescence polarization
Fluorescence polarization (FP) measurements are made using an optical system that includes polarizing filters in the light path. Samples in the microplate are excited using polarized light. Depending on the mobility of the fluorescent molecules found in the wells, the light emitted will either be polarized or not.
Learn MoreTR-FRET
Time-resolved fluorescence energy transfer (TR-FRET) works on the principles that when suitable pairs of fluorophores are in close proximity of one another, excitation of a lanthanide donor fluorophore results in energy transfer to an acceptor fluorophore.
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Microplate Readers - FAQs
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