xCELLigence RTCA CardioECR - Cardiomyocyte Contractility & Electrical Activity
The CardioECR can also be used to functionally mature hiPSC cardiomyocytes with its electronic pacing function. Paced hiPSC cardiomyocytes provides a significantly improved cell models used in various applications including safety/tox assessment, drug discovery, and cardiac disease research.
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Product Details
- Monitor excitation-contraction coupling and integrated ion channel activity
- Generate data that highly correlates with arrhythmogenic activity
- Use the pacing function for more tightly controlled assays
- Obtain a more thorough understanding of drug mechanism of action
Cellular Impedance Explained
The functional unit of a cellular impedance assay is a set of gold microelectrodes fused to the bottom surface of a microtiter plate well. When submersed in an electrically conductive solution (such as buffer or standard tissue culture medium), the application of an electric potential across these electrodes causes electrons to exit the negative terminal, pass through bulk solution, and then deposit onto the positive terminal to complete the circuit.
Learn More- Application Notes
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Cardiotoxicity Assessment of Antiviral Drugs Using the Agilent xCELLigence RTCA CardioECR System
This study used the Agilent xCELLigence RTCA CardioECR system with human iPSC-derived cardiomyocytes (hiPSC-CMs) to evaluate cardiac safety/toxicity of antiviral drugs.
- Application Notes
- English
- 21 May 2024
- 1.55 MB
Multimodal Connected Workflow for Cardiotoxicity Assessment Using Human iPSC-Derived Cardiomyocytes
This application note demonstrates the feasibility and use of combining multiple cell-based in vitro assays from Agilent that use diverse analytical technologies to investigate the cardiac liability of drugs.
- Application Notes
- English
- 26 Sep 2022
- 1002.14 KB
Connected Workflow: Assessment of Inotropic Compounds in PacedCardiomyocytes Using Calcium Imaging
This app note demonstrates the feasibility of using a connected workflow to evaluate the effect of inotropic compounds on electrically paced human iPSC derived cardiomyocytes (hiPSC-CMs) through calcium imaging
- Application Notes
- English
- 06 Jun 2022
- 2.93 MB
Assessment of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocyte Disease Models Using the Agilent xCELLigence CardioECR System
A demonstration of the use of the Agilent xCELLigence RTCA CardioECR system to compare functional profiles of contractility and electrophysiology between an PS-iPSC-CM disease model and its isogenic control.
- Application Notes
- English
- 10 Sep 2021
- 2.82 MB
Using the Agilent xCELLigence RTCA ePacer for Functional Maturation of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes
This application note presents a method for improving human-induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) maturation using the Agilent xCELLigence RTCA ePacer system.
- Application Notes
- English
- 11 Feb 2021
- 5.39 MB
A Real-Time Impedance Analysis Instrument for Cardiac Safety and Toxicity Assessment
In this application note, the RTCA Cardio instrument is used with human stem cell-derived cardiomyocytes (iCell Cardiomyocytes) for assessment of the cardiac safety profile of leading compounds or drug candidates during drug development.
- Application Notes
- English
- 04 Dec 2020
- 2.01 MB
The Agilent xCELLigence RTCA CardioECR System: A Multiparametric Functional Analysis Instrument for Cardiac Safety and Toxicity Assessment
In this application note, we used the xCELLigence RTCA CardioECR system (CardioECR system) in conjunction with hiPS-CMs for the assessment of preclinical cardiac risks.
- Application Notes
- English
- 16 Jul 2020
- 4.82 MB
- Article Reprints
- Brochures
- Data Sheets
- User Manuals
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User Guide for iCell Cardiomyocytes Assay on an xCELLigence RTCA CardioECR System
A guide for using the xCELLigence RTCA CardioECR system to analyze iCell cardiomyocytes.
- User Manuals
- English
- 21 Aug 2020
- 148.23 KB
E-Plate Coating User Guide
A guide for coating E-Plates.
- User Manuals
- English
- 21 Aug 2020
- 120.50 KB
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