产品详细信息
- 更宽的分子量测定范围 — 在约 1.5 小时内分离高达 165 kb 的高分子量 DNA 弥散条带和片段。
- 飞克级灵敏度 — 可检测低至 50 fg/µL 的核酸，核酸弥散条带的灵敏度提高至 10 倍，核酸片段的灵敏度提高至 100 倍。
- 精简的工作流程 — 利用自动化毛细管电泳取代脉冲场凝胶电泳，可加快长读长 NGS 文库制备和 BAC 分析。
- 样品前处理简单 — 较少的移液步骤和稳定的试剂，大幅减少样品降解。
- 样品容量大 — 可同时容纳多达三个 96 孔样品盘，能够对多达 288 个样品进行无人值守分离。
- 所需样品量少 — 仅需 2 µL 珍贵样品，能够以优异的灵敏度实现出色的检测。
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- 重要资料
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Visualizing DNA for Long-Read Sequencing by Moles, Not Mass
Visualization of the sample by moles using the Femto Pulse system can provide a clearer assessment of the length distribution, and better predict sequencing results.
- 应用简报
- English
- 2024 年 4 月 7 日
- 645.41 KB
Bacterial Artificial Chromosome Analysis on the Agilent Femto Pulse System
The Agilent Femto Pulse system was successfully employed as a screening tool for BAC quality by providing faster visualization and sizing of digested BAC DNA fragments.
- 应用简报
- English
- 2023 年 5 月 8 日
- 610.75 KB
Comparison of Constant- and Pulsed-Field Electrophoresis Technologies for Analysis of High Molecular Weight and Large DNA Fragments
This application note demonstrates the use of the Agilent Fragment Analyzer and Femto Pulse systems for analysis of high-molecular-weight and large DNA fragments.
- 应用简报
- English
- 2023 年 6 月 22 日
- 540.88 KB
- 应用简报
- 产品样本
- 案例研究
- 数据表
- 宣传单页
- 技术概述
- 常见问题解答
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- 用户手册
Ferris Jung
Research Technician, EMBL Genomics Core Facility
The Femto Pulse helps us to reliably evaluate the quality of genomic DNA in an easy and straight forward way. Since the quality of the input material is the bottle neck of NGS applications, it gives us the possibility to optimize our protocols effectively, especially for applications like Oxford Nanopore.
查看更多Dr. Bruno Huettel
Max Planck-Genome-centre Cologne
The Femto Pulse definitely filled an existing gap in quality control (QC) for RNA and DNA. Especially from chromatin immune precipitated (ChIP) samples it was very difficult to draw some conclusions in QC with previous QC methods because of the very low amounts (low picogram range) usually provided by the user/customers. The pulse field function of the Femto Pulse makes it a very useful tool to judge on the quality of input samples and libraries for Nanopore and PacBio sequencing.
查看更多Dr. Wassim Lakhal
Team Leader in the Equipments Department, Hologic Diagenode
I couldn’t imagine how our daily lives in the lab would be without the Femto Pulse instruments, or how we would be advancing in our different projects. It came in at the perfect time. It takes five to 15 minutes to prepare the instrument and then the rest is really flexible, so we can run a lot of samples. Also, the ProSize is quite an amazing software. We can combine samples, superimpose, compare, change the scales, and play with the baseline.
查看更多