DNF-472-0500
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Fragment Analyzer RNA Kits

Part Number:
DNF-472-0500
RUO

HS RNA Kit (15NT), 500. Fragment Analyzer system kit for the quantitative and qualitative analysis of total RNA and IVT mRNA from 200 to 6,000 nt at low and mid concentrations using the standard methods or IVT mRNA through 9,000 nt with the extended methods. Includes reagents necessary for running 500 samples.​

For Research Use Only. Not for use in diagnostic procedures.
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List Price:
$1,686.00 /1 Kit

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Specifications
Analysis Time
  • Short array: IVT mRNA 45 min; IVT mRNA Extended method 90 min; Total/ribo-depleted RNA 40 min
Assay Sensitivity
  • High
Guaranteed Shelf Life
  • Minimum 4 months
Quantitative Accuracy
  • IVT mRNA: N/A​; Total/ribo-depleted RNA: ± 30 %
Quantitative Precision
  • IVT mRNA: N/A​; Total/ribo-depleted RNA:20 % CV
Quantitative Range
  • IVT mRNA Low Concentration: 500 - 2500 pg/µL; IVT mRNA Mid Concentration: 2500 - 10000 pg/µL; Total RNA: 50 - 5000 pg/µL; Ribo-depleted RNA: 500 - 5000 pg/µL
Sample Volume
  • 2 µL
Sensitivity
  • IVT mRNA Low Concentration: 15 pg/µL; IVT mRNA Mid Concentration: N/A; Total RNA: 50 pg/µL; Ribo-depleted RNA: 250 pg/µL
Sizing Accuracy
  • IVT mRNA: ± 15 %; Total/ribo-depleted RNA: ± 20 %
Sizing Precision
  • IVT mRNA Low Concentration: 10 % CV; IVT mRNA Mid Concentration: 5 % CV; Total/ribo-depleted RNA: 20 % CV
Sizing Range
  • 200 - 6000 nt; IVT mRNA with Lonza RNA Marker and Extended method: 500 - 9000 nt
Uses Per Kit
  • 500
Kit Contents
Part Number Product Description Quantity
DNF-265-0240 RNA Separation Gel, 240mL 1
DNF-301-0008 BF-1 Blank Solution, 8mL 1
DNF-355-0125 5x 930 dsDNA Inlet Buffer, 125mL 1
DNF-370-0004 HS RNA Diluent Marker (15 nt), 4 mL 3
DNF-386-U015 HS RNA Ladder, 15 µL 1
DNF-475-0050 5x Capillary Conditioning Solution, 50mL 1
DNF-497-0250 0.25x TE Rinse Buffer, 250mL 1
DNF-600-U030 Intercalating Dye, 30 µL 1
Support
User Manuals

Agilent DNF-472 HS RNA (15 nt) Kit Total RNA Quick Guide for Fragment Analyzer Systems

This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The DNF-472 HS RNA kit is designed to analyze Total RNA samples at an input concentration of 50 pg/μL – 5,000 pg/μL and ribo-depleted RNA at an input sample concentration of 500 pg/μL – 5,000 pg/μL . A separate quick guide (D0117260) covers the use of the DNF-472 HS RNA kit for analyzing in vitro transcribed (IVT) mRNA.

  • User Manuals
  • English
  • 24-Sep-2024
  • 385.74 Kb
  • PDF

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