DNF-464-0500
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Fragment Analyzer Large Fragment Analysis Kits

Part Number:
DNF-464-0500
RUO

HS Large Fragment 50kb Kit, 500. Fragment Analyzer system kit for the analysis of large DNA fragments and smears ranging from 75 to 50000 bp. Includes reagents necessary for running 500 samples.

For Research Use Only. Not for use in diagnostic procedures.
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List Price:
$1,686.00 /1 Kit

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Specifications
Analysis Time
  • Short array 55 min
Assay Sensitivity
  • High
DNA Sizing Range
  • 75 - 48500 bp
Guaranteed Shelf Life
  • Minimum 4 months
Kit Type
  • Quantitative
Qualitative Range
  • DNA Fragment: 5 – 600 pg/µL; DNA Smear; 50 - 5000 pg/µL
Quantitative Accuracy
  • ± 25 %
Quantitative Precision
  • 20 % CV
Sample Volume
  • 2 µL
Sizing Accuracy
  • DNA Fragment <15kb at 600 pg/μL and DNA smears at 1 ng/μL prepared from 1X TE buffer : ± 15 %
Uses Per Kit
  • 500
Kit Contents
Part Number Product Description Quantity
DNF-220-0240 Large Fragment Separation Gel, 240mL 1
DNF-300-0008 BF-25 Blank Solution, 8mL 1
DNF-355-0125 5x 930 dsDNA Inlet Buffer, 125mL 1
DNF-365-U125 HS Extended Large Frag DNA Ladder, 125uL 1
DNF-381-0003 HS Large Fragment Diluent Marker, 2.4 mL 5
DNF-475-0050 5x Capillary Conditioning Solution, 50mL 1
DNF-495-0060 Dilution Buffer 1x TE, 60mL 1
DNF-497-0125 0.25x TE Rinse Buffer, 125mL 1
DNF-600-U030 Intercalating Dye, 30 µL 1
Support
User Manuals

Agilent DNF-464 HS Large Fragment Kit Quick Guide for Fragment Analyzer Systems

This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The HS Large Fragment 50 kb kit (500 Samples) (Part # DNF-464-0500) is designed for the sizing and quantification of medium to high molecular weight dsDNA smears/fragments.