DNF-492-1000
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Obsolete

Part Number:
DNF-492-1000
RUO

Obsolete. Replaced by DNF-492-0500. Fragment Analyzer system kit for the quantitative and qualitative analysis of large DNA fragments and smears from 50 to 20000 bp. Includes reagents necessary for running 1,000 samples.

For Research Use Only. Not for use in diagnostic procedures.
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Specifications
Analysis Time
  • Short array 50 min
Assay Sensitivity
  • Standard
DNA Sizing Range
  • 75 - 20000 bp
Fragment Sensitivity Range
  • 0.1 - 10 ng/µL
Guaranteed Shelf Life
  • Minimum 4 months
Kit Type
  • Quantitative
Quantitative Accuracy
  • ± 25 %
Quantitative Precision
  • 20 % CV
Sample Volume
  • 2 µL
Sizing Accuracy
  • ± 15 %
Smear Sensitivity Range
  • 5 - 100 ng/µL
Uses Per Kit
  • 1000
Kit Contents
Part Number Product Description Quantity
DNF-220-0500 Obsolete 1
DNF-302-0008 BF-2000 Blank Solution, 8mL 1
DNF-355-0300 5x 930 dsDNA Inlet Buffer, 300mL 1
DNF-378-U100 Large Fragment DNA Ladder, 100uL 2
DNF-380-0003 Large Fragment Diluent Marker, 2.4 mL 10
DNF-475-0100 5x Capillary Conditioning Solution, 100 mL 1
DNF-497-0125 0.25x TE Rinse Buffer, 125mL 1
DNF-600-U030 Intercalating Dye, 30 µL 2
Support
User Manuals

Agilent DNF-492 Large Fragment Kit Quick Guide for Fragment Analyzer Systems

This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The Large Fragment kit (p/n DNF-492-0500 and DNF-492-1000) is designed for the sizing and quantification of small to mid-range molecular weight dsDNA smears/fragments. Example applications include quality control of Next Generation Sequencing (NGS) libraries.