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Agilent BioTek microplate readers are designed to deliver the best results across many different detection modes and far-ranging applications. Other unique technologies further enhance performance for specific workflow requirements. Learn more about the technologies used in our multimode readers and single mode readers here.
Absorbance refers to the ability of a substance to absorb light of a specific wavelength.
AlphaScreen is a bead-based assay technology used to study biomolecular interactions in a microplate format.
Filters can be used to modulate the luminescence emanating from the sample.
Flash luminescence is the result of a chemical or biochemical reaction, triggered by a rapid injection of a catalyst into the microplate well immediately before detection.
Fluorescence intensity detection has a much broader range of applications than absorbance detection.
Fluorescence polarization (FP) measurements are made using an optical system that includes polarizing filters in the light path.
Time-resolved fluorescence (TRF) measurement is similar to fluorescence intensity (FI) measurement.
Time-resolved fluorescence energy transfer (TR-FRET) works on the principles that when suitable pairs of fluorophores are in close proximity of one another, excitation of a lanthanide donor fluorophore results in energy transfer to an acceptor fluorophore.
Many Agilent single and multi-mode microplate readers include an incubation function for temperature sensitive applications, such as kinetic assays.
At one time, scientists had to make an important decision when purchasing a multidetection microplate reader: Do I need filter-based or monochromator-based optical system? With the Agilent BioTek patented Hybrid Technology, there is no need to choose.
The Peltier cooling module for the Agilent BioTek Cytation 7, 5, and 1 cell imaging multimode readers helps maintain temperature stability inside the reading chamber. The temperature is stable regardless of changes in ambient (room) temperature and heat produced by electro-mechanical operations within the reader.
Many multimode microplate readers use monochromator-based optics for fluorescence intensity measurements, allowing great flexibility to use multiple fluorescent dyes and stains.
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