Skip to main content

Store High Sensitivity RNA sample buffer and ScreenTape devices: 2 – 8 °C (36 – 46 °F).

Store High Sensitivity RNA ladder at -20 °C to -5 °C (-4 °F to 23 °F).

Store partially used ScreenTape devices upright at 2 – 8 °C (36 – 46 °F) for a maximum of 2 weeks.

Never freeze ScreenTape devices. Discard any accidentally frozen ScreenTape devices.

The RIN algorithm from the 2100 Bioanalyzer system uses a neural-trained network for determining the RNA quality.

The RINe algorithm in the TapeStation assays considers the peak height of the 18S peak in relation to the background signal to calculate a RINe value for total RNA.

Yes. However, due to possible additional rRNA peaks from the plastids an internal threshold for the RINe needs to be established.

Yes. However, the RNA assay is designed to assess quantity and integrity of total RNA samples. The lower molecular weight range is not sufficient to analyze small RNA in good resolution. Small RNA runs close or into the lower marker peak. Consider using the Small RNA kit of the 2100 Bioanalyzer system.

For best sizing precision and accuracy, a ladder per ScreenTape device is recommended. Alternatively, an electronic ladder is also available, the electronic ladder can be selected in the TapeStation Controller Software and is added to the data automatically.

More detailed troubleshooting information on hardware, software and application issues can be found in the TapeStation System Manual or in the Agilent Information Center, which is part of the TapeStation software package.

To obtain a Certificate of Conformance (CoC) for ScreenTape reagents, scan the QR code on the product label with your phone or type the lot number into the search field of the Agilent homepage.