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Next-generation sequencing (NGS) is an essential technology in the modern genomics workflow allowing for massively parallel sequencing. Library preparation for NGS is a standardized procedure which generally involves fragmentation of the input material and ligation of functional adapters to the DNA fragments which can then be analyzed on the respective sequencer. Several NGS library preparation steps must be correctly performed to ensure high-quality data for downstream analysis. The library preparation workflow, while critical, is also time-consuming, labor-intensive, and expensive. Implementing NGS QC within library preparation protocols enables laboratories to conserve valuable time and resources while ensuring successful sequence data. Agilent offers automated electrophoresis systems for accurate, efficient, and reliable quality assessment of NGS libraries. Discover how sample QC solutions can advance clinical research, translational research, and molecular biology projects.

Sample QC steps  |   Sample quality metrics  |   Data examples   |   Resource center

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Sample QC enhances NGS library preparation quality

Three steps to access NGS sample quality

Watch the three-part webinar series to learn about input sample QC, overall library QC, and to explore sample QC examples from subject matter experts.

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The role of quality metrics in library prep QC

QC metrics are reliable, objective measures of sample integrity. Learn about quality control for sample types such as gDNA, cfDNA, RNA, FFPE, and more.

Explore the metrics

Explore selected application examples and data

Explore selected QC data examples that show high resolution separation, sizing, and quantification from gDNA, small RNA, total RNA and other sample types.

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Looking for expert insights on NGS QC methods, technologies and solutions?

Our wide variety of on-demand webinars, presented by industry experts and Agilent product specialists, cover many relevant topics in NGS sample QC and highlight how Agilent NGS solutions help provide you support. 

Find the webinars best suited to your needs and register today to advance your NGS.



White paper reviews several applications for sample QC in NGS library prep

Twelve research labs achieved NGS success by adding sample QC to their library prep processes. They share their findings in this white paper, which include:

  • Monitoring sample quality in real time to make informed decisions about proceeding through the workflow
  • Maximizing library prep with effective sample QC for unique sample libraries (such as cell-free DNA) can minimize challenges
  • Adding quality testing for more consistent library preparations

More resources for NGS sample QC

We have compiled additional reference materials to help you explore automated electrophoresis and NGS library prep quality control. Whether you are working in molecular biology research, translational research, or clinical research, these references can help you find and implement tailored QC solutions. Search through interesting resources such as:

  • Curated literature
  • Selected videos
  • Interactive guides

Complete NGS solutions from sample to report

Our NGS solution also includes the Agilent SureSelect portfolio with comprehensive library preparation kits, the Agilent Bravo or Magnis NGS Prep System for automation and the Alissa Clinical Informatics Platform for interpretation and reporting, delivering you the flexibility you need to work with your sample type while providing you confidence in your results.
 
No matter your application, sample type, or sample quantity, we offer the tools and support you need throughout your journey.



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For Research Use Only. Not for use in diagnostic procedures.

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