Skip to main content

Investigate Dynamic Cancer Cell Strategies with Innovative Cell Analysis Technologies

In recent years, the cancer treatment landscape has rapidly evolved with the adoption of novel immuno-oncology and cell-based therapies. The development of safe and effective therapies requires a deeper understanding of cancer cell biology, enabled by effective cell analysis tools and products for investigating cancer cell biology and the complex tumor microenvironment.

Contact us
×

Contact Us


Cancer Research Areas

Cell Migration and Invasion

Capture critical processes related to cell invasion and migration to understand the role of metastasis in cancer progression.

Learn More

Cell Health and Viability

Generate detailed profiles of cancer cell proliferation and viability over time to gain insight into cellular behavior driving the disease process.

Learn More

Cell Cycle Analysis

Capture critical processes related to cell migration and invasion the role of metastasis in cancer progression

Learn More

Cancer Metabolism

Reveal cancer metabolic liabilities through functional bioenergetic analysis of live cells in real-time for a deeper understanding of cancer cell biology.

Learn More

Hypoxia Research

Monitor cancer cell behavior under hypoxic conditions to more accurately represent the oxygen-restricted microenvironment within a tumor.

Learn More

Cell Quality Control

Assess quality of cancer cell lines and identify phenotypic changes caused by cell culture conditions.         

 

Learn More

Cell Adhesion

Understand the intricacy of metastasis and the kinetics of adhesion of different cell types, such as circulating tumor cells.

Learn More

Cell-to-Cell Interactions

Investigate cell-cell interactions within the tumor microenvironment to reveal the mechanisms behind cancer initiation, promotion, and immune evasion.

Learn More

Immuno-Oncology

Dive deeper into immune cell function and real-time cell and gene therapy live cell assays.

Learn more

Cell Barrier

Monitor cell barrier function disruption and recovery to unmask more kinetic details in cancer metastasis and progression.

Learn More

Advancements in Automated Confocal Imaging

Water immersion objectives with high numerical apertures (NA) collect more light with lower exposure times, and do not exhibit the distortion that can occur with air objectives. These advantages and others inherent to water immersion imaging, result in higher quality images. In this application note, we describe using water immersion objectives with the Cytation C10 confocal imaging reader to obtain high quality images and data from 2D and 3D samples.


3D Spheroid-Based Tumor Invasion Assay

Tumors in vivo exist as a three-dimensional (3D) mass of multiple cell types, including cancer and stromal cells. Therefore, incorporating a 3D spheroid-type cellular structure that includes cocultured cell types forming a tumoroid, provides a more predictive model than the use of individual cancer cells seeded in microplates. The BioTek BioSpa live cell analysis system supports advanced long-term imaging studies of 3D cellular structures, including characterization of 3D spheroid-based tumor invasion assays.


Detecting Circulating Tumor Cells by Flow Cytometry

Circulating tumor cells (CTC) are rare cells shed from a solid tumor or metastases that circulate through the bloodstream. Flow cytometry can be used to detect CTC as a biomarker for metastatic cancer research and drug discovery.  However, detection of CTC poses a challenge because they are present at extremely low concentrations.

Designed for both novice and expert users, the highly sensitive Agilent NovoCyte flow cytometers are intuitive and easy to use for CTC studies and other cancer research applications.


Targeting Cancer Bioenergetics using Seahorse XF Technology

Altered energy metabolism is now recognized as a driver in cancer metastasis, with metabolic intermediates emerging as promising therapeutic targets. Agilent Seahorse XF technology provides critical functional measurements in live cells in real-time for potential drug target identification and validation. By measuring bioenergetic phenotype, cellular ATP production rate, mitochondrial and glycolytic function, and substrate dependencies, cancer metabolic vulnerabilities can be revealed to advance therapeutic discovery.



Automated Colony Formation Assay

The colony formation assay, or clonogenic assay, evaluates the proliferative capacity of a single cell—an important factor in characterizing cancer models and evaluating treatment effects. Conventional colony formation assay formats are manual and rely on subjective scoring. The automated imaging colony formation assay developed for the Agilent Lionheart automated microscope and Agilent Cytation cell imaging multimode readers utilizes powerful image analysis tools for accurate and reproducible determination of colony counts.


Real-Time Cell-Cell Interactions ​

Monitoring of T47D cellular response to H295R estrogen signaling is shown. Estrogen and progesterone receptor positive T47D breast cancer cells were grown on xCELLigence E-plates. A coculture insert containing H295R adrenal corticocarcinoma cells was placed on the E-plate. The response of the T47D cells to the secreted hormones was suppressed by the application of estrogen synthesis inhibitors, ketoconazole and anastrazole, to the stimulatory H295R cells.


Real-Time Cell Adhesion Assay

Monitoring of T47D cellular response to H295R estrogen signaling is shown. Estrogen and progesterone receptor positive T47D breast cancer cells were grown on xCELLigence E-plates. A coculture insert containing H295R adrenal corticocarcinoma cells was placed on the E-plate. The response of the T47D cells to the secreted hormones was suppressed by the application of estrogen synthesis inhibitors, ketoconazole and anastrazole, to the stimulatory H295R cells.



High-Throughput Imaging of 3D Engineered Model Constructs

This webinar delivered by the Tilbury Lab, discusses high-throughput, rapid screening methodologies for 3D models using the Cytation 5, including:​

  • Developing a collagen degradation model to explore the relationship between collagen remodeling in the ECM and a collagen cryptic α10 integrin binding site that modulates cellular behavior
  • Exploring weak autofluorescent signals of NAD(P)H and FAD to probe the metabolic and proliferation states of cancer spheroids
  • Characterizing cellulose nanofibers as a potential biomaterial for bone regrowth

Imaging and Analysis of Spheroids to Determine Dose Response During Drug Treatment

Spheroids models are increasingly used to represent the complex 3D cancer microenvironments. The ability to automate image capture using multiple imaging modes and perform automated image analysis can significantly increase throughput and allow for comparative analysis. 

This application note describes the use of an automated confocal imaging assay for accurate determination of cell number in spheroids for evaluation of drug dose response using the Agilent BioTek Cytation C10 confocal imaging reader.




For Research Use Only. Not for use in diagnostic procedures.

×

Contact Us