Agilent CGH Assay Workflow
Agilent's high quality, oligonucleotide library synthesis (OLS) based CGH microarrays accurately detect copy number variations at the whole genome level. Unlike other SNP-based, chromosomal microarray analysis (CMA) platforms, Agilent offers a true 2 color comparison between validated, biological reference DNA and sample. Agilent's CGH microarrays are manufactured with proprietary (OLS) technology, which produces high quality 60 mer long oligos optimized to precisely target any region of interest with base pair resolution. Agilent's aCGH platform features a combination of optimal probe length and probe coverage to accurately detect small differences in copy number changes.
Agilent Custom CGH+SNP Microarray product line uses Agilent's SurePrint technology to design and print user-defined CGH+SNP microarrays. Using Agilent's eArray free application, users can design their own CGH+SNP microarrays to detect copy number changes and copy neutral aberrations, such as loss of heterozygosity (LOH) and uniparental disomy (UPD), on the same array.
Agilent CytoGenomics provides a statistically and visually powerful tool to streamline the day-to-day cytogenetic sample analysis research workflow. It offers full support for efficient analysis of cytogenetic samples and easy report generation, based on information from both external databases and an internal database. These self-paced tutorials help in better understanding of how to best use this software in your research.
The Agilent microarray workflow is streamlined and takes less time to process samples compared with other methods. Agilent offers complete solutions with reagents and instruments needed to process your aCGH data including custom and catalog arrays, labeling kits, hybridization and wash solutions, hybridization oven, the SureScan microarray scanner, and easy-to-use software to interpret your results.
Streamlined data analysis
CytoGenomics can analyze CGH data for RUO
Two-color platform with new improved dyes for light and ozone tolerance
Short workflow without PCR
Complete experiment in small space — no special room required
Cancer, Prenatal and Postnatal, IVF
High quality 60-mers
Sample to biological reference vs insilico comparison
Mosaicism detection down to 8%
For Research Use Only. Not for use in diagnostic procedures.