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DV200 Analysis with the Agilent Bioanalyzer system

DV200 Analysis with the Agilent Bioanalyzer system
Quickly determine the quality of your FFPE RNA samples with the Automated Electrophoresis Portfolio

The Automated Electrophoresis systems from Agilent utilize a quality metric, DV200, to address the unique QC challenges of FFPE RNA. The DV200 provides researchers with a tool to reliably classify degraded RNA by size and effectively parse samples suitable for NGS from unsuitable samples, conserving time and costs. Depending on the level of degradation and the DV200 score, researchers can modify their workflows to still enable the use of FFPE samples in downstream applications. For example, when a specific size is needed and the sample is slightly degraded, the shearing protocol can be adjusted to reach that size. Take advantage of automated evaluation of RNA samples using the DV200 quality metric with the Bioanalyzer system

2100 Bioanalyzer system

If you are not using the 2100 Expert software revision B.02.10 or higher, please download the DV200 assay files:

Extract and store the DV200 RNA Nano.xsy or DV200 RNA Pico.xsy file in the 2100 Expert Software RNA assay folder:
c:\Program Files (x86)\Agilent\
2100bioanalyzer\2100expert\assays\RNA

To apply the DV200 calculation to an existing data file:

  1. Open the data file with the 2100 Expert Software
  2. Navigate to the Assay Properties tab
  3. Click Import Setpoints and select the appropriate .xsy file (DV200 RNA Nano or DV200 RNA Pico, dependent on the data file opened)

Assay parameters will be applied to the data file. Refer to the Technical Overview for more details.

Total RNA sample on the Bioanalyzer

For Research Use Only. Not for use in diagnostic procedures.