The Fluorescence-Based Aggregation Analysis Workflow Guide
High-Throughput Fluorescence-Based mAb Aggregation Analysis Solution
The Fluorescence Workflow solution for rapid mAb aggregation analysis is a documented method that has been optimized by experts in the field to screen high sample loads common in clone selection and formulation optimization experiments. The workflow manual has been designed for the Cary Eclipse Fluorescence instrument fitted with the Microplate Reader Accessory and is perfectly suited to complement existing HPLC-SEC workflows. The method includes the step-by-step instructions to implement the complete workflow.
With superior speed and sensitivity, this workflow can reduce overall analysis time by up to 75% when used in conjunction with existing HPLC-SEC methods, allowing significant improvements in both cost and throughput.
With superior speed and sensitivity, this workflow can reduce overall analysis time by up to 75% when used in conjunction with existing HPLC-SEC methods, allowing significant improvements in both cost and throughput.
Product Details
Features
- Get estimates of high-molecular weight aggregation in approximately 5 seconds per sample.
- Fast monoclonal antibody aggregation analysis.
- Add to existing HPLC-SEC chromatography workflows for time savings of up to 75%.
- mAb clone selection based on extrinsic fluorescence of PEPBOPS dye.
- High sensitivity screening for aggregation using specific dyes.
- Have confidence in data with an established method optimized by aggregation experts.
- High throughput.
- Sensitivity and specificity - dye only binds to aggregated samples, and does not contribute to background signal.
- No column washing or equilibration required.
- Minimize method development time.
