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Agilent Seahorse XF Instruments Overview and Selection Guide

Agilent Seahorse XF Instruments
Overview
Selection Guide

Overview

All Seahorse XF instruments analyze mitochondrial respiration and glycolysis in live cells, generating data in just minutes. Seahorse XF technology uses solid-state sensors to simultaneously measure both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in every well. All platforms support injection of up to 4 unique compounds per well.

 
Agilent Seahorse XF Pro Analyzer
XF Pro Analyzer

The Seahorse XF Pro Analyzer provides maximum capacity for XF assays at lowest cost-per-sample with high-sensitivity capability, verified instrument performance, repeatability specifications, and optimized temperature control. The Seahorse XF Pro enables many experimental groups per assay, maximizing design flexibility and sample throughput. It comes with state-of-the-art software and streamlined workflows for enhanced user experience.
Agilent Seahorse XF HS Mini Analyzer
XFp

The Seahorse XF HS Mini analyzer is equipped with high-sensitivity capabilities – combined with an easy-to-use interface. This makes this eight-well analyzer accessible for every lab while delivering robust XF analysis on limited cell numbers.
Agilent Seahorse XFe24 Analyzer
XFe24

The Seahorse XFe24 is validated by hundreds of peer-reviewed publications, and utilizes a larger well format which is suitable for islets and other specialty sample types. Validated for assays performed in a hypoxia chamber.
 

Selection Guide

 

  Agilent Seahorse XF Pro Analyzer Agilent Seahorse XF HS Mini Analyzer Agilent Seahorse XFe24 Analyzer
Plate Format Microplate Miniplate Microplate
Number of Assay Wells 96 8 24
Microchamber volume 2.28 μL 2.28 or 0.71 μL 5.65 μL
Assay Working Volume 150–275 µL 150–275 µL 500-1000 µL
Recommended Injection Volume 25 µL 25 µL 75 µL
Controller Combination computer and touch screen display with full assay design, control, and analysis capability. Integrated full-color touch screen interface to design basic protocols and run imported templates. Combination computer and touch screen display with full assay design, control, and analysis capability.
Software Wave Pro controller software includes preloaded templates for quick experimental design, automatically calculates rates in real time, and provides exclusive cutting-edge data analysis tools (e.g., multifile analysis, dose-response curves, Z'), advanced template import feature and dose-response experiment set Create and run standard assays on controller and perform data analysis with Seahorse Analytics. Design and analyze assay templates on Controller or Wave desktop.
Best For
  • Phenotypic screening
  • Testing many conditions at once
  • Dose-response studies
  • Spheroids
  • Pairwise comparisons
  • Phenotyping single samples
  • Precious biomaterial
  • Ex-vivo and primary cells
  • Islets
  • Larger samples
Key Advantages
  • High sensitivity
  • Tight error in low ranges down to OCR of 13 pmol/min
  • Verified instrument performance
  • Advanced thermal control to reduce edge effect when used with XF Pro M plates
  • Automated data QC
  • IQ and AIQ certified instrument performance experimental flexibility
  • Highest throughput
  • Tested for hypoxia
  • Support for assay temperatures 16–42°C (12–20°C lower ambient temperature required)
  • Easy to set up and run via streamlined interface
  • Standardized protocols for XF Assays
  • Support for assay temperatures 16-40°C (8–20°C lower ambient temp required)
  • Tested for hypoxia
  • Support for assay temperatures 16–42°C (12–20°C lower ambient temperature required)
  • Balances throughput and budget considerations

The Agilent Seahorse XF analyzers feature:

 

See more information on How Agilent Seahorse XF Analyzers work.

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For Research Use Only. Not for use in diagnostic procedures.