Rapid Seahorse XF methods that measure mitochondrial substrate oxidation
Long-chain fatty acids (LCFAs), glucose/pyruvate and glutamine are three primary substrates that fuel mitochondrial metabolism. Researchers in many areas (e.g., cancer, immunology, stem cell biology) have demonstrated how oxidization of these substrates has profound impact on cell fate, function, and fitness. Many of the findings not only provide new biological insights, but also reveal novel approaches to intervention and developing successful research therapies. The Seahorse XF Substrate Oxidation Stress Test Kits enable rapid assessment of substrate oxidation in live cells.