Solid Phase Extraction (SPE) Cartridges, Well Plates, Sorbents

Solid Phase Extraction (SPE) is a sample preparation technique with principles similar to those of high-performance liquid chromatography (HPLC) for selective adsorption of analytes or interferences from complex matrices. SPE extraction is used for sample clean-up and analyte concentration preceding liquid chromatography (LC), gas chromatography (GC), ion chromatography and other techniques. SPE is considered a cost-effective alternative to or replacement for liquid-liquid extraction (productivity, solvent, waste).

The advantages of SPE versus liquid-liquid extraction include improved throughput, decreased organic solvent usage and waste generation, higher and more reproducible recoveries, cleaner extracts, no emulsions, tunable selectivity and automation.

Typical applications of SPE include sample clean-up, trace enrichment, desalting, solvent exchange and sample preservation and storage.

SPE usage is accelerating in combinatorial synthesis and high-throughput LC-MS analysis. Increase in overall LC-MS usage improves instrument uptime. SPE decreases organic solvents and waste.

Agilent offers SPE products in various polarities and chemistries to achieve consistent sample processing. A broad selection of Bond Elut sorbents are available in cartridge and well plate formats and as bulk sorbents. Frits, adapter caps, empty cartridges for custom packing, and online SPE cartridges can also be purchased. Bulk packaging of SPE products provides a cost-effective solution for high-throughput workflows.