NovoSampler Pro
Walk away convenience delivers on time savings and cost savings in the laboratory. Flexible sampling of microplates allows for small projects or high-throughput, 24/7 workflows. Low carryover, and orbital shaking allow you to maximize your efficiency and make the instrument work for you.
- Flow Cytometry Automation
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Product Details
- Trusted results with low carryover – <0.1% (volume)
- Convenient automated sample mixing – Orbital shaking up to 3000 rpm
- Versatile workflows are compatible with 24-, 48-, 96-well plate, and 24 tube racks
- Walk away sample acquistion
- Save time by making the instrument work for you
Delivers consistent data across different loading templates
Apoptosis Assay
Apoptosis, or programmed cell death, is the process by which cells regulate how they die, activating specific pathways that cause the cell to shrink, condense, and eventually be cleared by phagocytosis. This is in contrast to necrotic cell death where cells die uncontrollably and fall apart, which can lead to detrimental effects such as the activation of an immune response. Therefore, apoptotic cells that die in a very orderly fashion limit disruption of nearby cells and tissue.
Immunophenotyping
Immune status is associated with disease state, treatment efficiency, and response to external stimuli such as vaccines. Immunophenotyping quickly identifies candidate cell types, sub-classes and functions. Monitoring the frequency of numerous immune cell population as well as the differentiation/activation status of specific cell subsets such as monocytes, NK cells, T and B cells is essential as they may influence the immunogenicity of a vaccine and its efficiency. The NovoCyte Flow Cytometer enables simultaneous quantification of multiple leukocytes for better understanding the immune status of patients and surveillance of the immune response to infectious disease.
Cell Proliferation
Cell proliferation is an essential function and highly structured event that when unregulated, can cause disease. We can measure proliferation through absolute cell counts or with a dye, such as CFSE. When cells labeled with CFSE divide, the dye is partitioned equally between daughter cells and we can measure the loss of CFSE fluorescence over time as the dye is continuously diluted. The mean fluorescence intensity (MFI) of the dye was also plotted with cell concentration over time to show the inverse relationship between the two. This type of assay is often used to look at changes in T lymphocyte activation.
Cytokine Detection
Cytokines are small molecules essential for immune cell response to activation by pathogens, autoimmunity, or therapeutics. Signaling by cytokines can modulate gene regulation, innate and adaptive immune response, and inflammation. Measuring cytokine production and identifying the source of cytokine production is therefore important for an in-depth understanding of the immune response. Bead-based flow cytometry-based detection of cytokines is a highly effective method for measuring multiple soluble analytes in a single sample, using a mixture of bead populations with varying fluorescence intensities.
Intracellular Protein Detection
Detection and analysis of intracellular proteins allow for additional characterization of cell subpopulations and cellular processes. In order to analyze proteins not located on the cell surface, fixation and permeabilization of the cell is required. However, many phospho-specific antibodies are not compatible with many common detergent-based permeabilization methods used for intracellular staining. Special attention is needed when determining the proper fix/perm method for your phospho-specific antibody. The most common method uses 1.5% paraformaldehyde for fixation followed by 100% methanol for permeabilization. While this method works for many antibodies, please note it may not work for every phospho-specific antibody.
Additionally, identifying various cell populations in a heterogenous sample requires staining for phosphorylated proteins coupled with surface proteins. Special consideration must be given to the sensitivity of these epitopes to fixative, taking precaution to avoid damage to the epitope. Therefore, the sample may require staining for specific surface markers before fixation.
Cell Cycle Analysis
Normal human somatic cells are diploids containing a constant amount of DNA. During cell cycle progression, DNA synthesis results in a doubling of total DNA content, followed by restoration of the normal DNA content after mitosis. Detailed cell cycle analysis can be performed to understand tumor cell differentiation, cell transformation and cell-compound interaction with the NovoCyte flow cytometer.
Figure: After treatment with 10 migrograms/M MG132 or 500 micrograms/M 5-FI for 16 hours/ A549 cells were analyzed for cell cycle distribution with the ACEA Novocyte flow cytometer. The the Novoexpress built-in cell cycle analysis module, the plot shows cells ni G0/G1 phase (green), S phase (yellow) and G2/M phase (blue). Compared to normal untreated cells, MG132 treated cells were arrested at G2/M phase, while 5-FU treated cells were arrested at G0/G1 phase.
- Application Notes
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HPV E6/E7 mRNA Expression Analysis Using the Agilent NovoCyte Flow Cytometer
A demonstration of the detection of mRNA for two HPV oncoproteins in clinical samples using the FLOWSCRIPT HPV E6/E7 assay and the Agilent NovoCyte flow cytometer.
- Application Notes
- English
- 02 Jul 2020
- 348.81 KB
- Flyers
- User Manuals
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NovoSampler Pro Operator's Guide (RUO)
NovoSampler Pro Operator's Guide (RUO)
- User Manuals
- English
- 04 Jun 2021
- 3.74 MB
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