pAAV-hrGFP Vector
By eliminating the requirement for live helper virus, the AAV Helper-Free System provides a safer, purer and more convenient alternative to retroviral and adenoviral gene delivery systems. In the AAV Helper-Free system, the AAV-2 ITR-containing plasmids (pAAV-MCS, pAAV-LacZ and pAAV-hrGFP and pAAV-IRES-hrGFP) do not share any regions of homology with the rep/cap-gene containing plasmid (pAAV-RC), preventing the production of wild-type AAV-2 through recombination. To ensure that this lack of homology is maintained, it is important to use all of the components provided with the AAV Helper-Free system together.