AAV-293 Cells
RUO
AAV-293 cells are an HEK293 cell line optimized for packaging of AAV virions in the helper-free system. AAV-293 cells, like HEK293 cells, produce E1 in trans. AAV-293 cells allow production of infectious virus particles when transfected with E1-deleted adenovirus vectors or co-transfected with the three AAV helper-free system plasmids.
These plasmids supply all of the trans-acting factors required for AAV replication and packaging in AAV-293 cells. Recombinant AAV-2 viral particles prepared from infected AAV-293 cells may then be used to infect a variety of mammalian cells.
These plasmids supply all of the trans-acting factors required for AAV replication and packaging in AAV-293 cells. Recombinant AAV-2 viral particles prepared from infected AAV-293 cells may then be used to infect a variety of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures.
Product Details
Features
- AAV-293 cells are derived from the commonly used HEK293 cell line but produce higher viral titers.
- HEK293 cells are human embryonic kidney cells transformed by sheared adenovirus type 5 DNA.
- Replication of the AAV-2 vector requires the adenovirus E1 gene product stably expressed in the HEK293-derived AAV-293 cells.
