The InterPlay Mammalian TAP System allows you to recover interacting proteins from mammalian cells. This method is based on expression of a protein of interest fused to two affinity tags: a streptavidin binding peptide (SBP) and a calmodulin binding peptide (CBP). Tandem affinity purification yields your tagged protein and interacting proteins using gentle washing and small molecule elution conditions.
The C- and N-terminal mammalian TAP vectors let you clone your gene of interest with the SBP and CBP tags fused to either the C- or N-terminus of your gene.
For Research Use Only. Not for use in diagnostic procedures.
InterPlay Mammalian TAP System instruction manual covering vector maps, preparation, mammalian cell transfection, TAP protocol, purified protein detection, troubleshooting, an