pERV3 Vector
The pERV3 vector is designed so that the genes for both VgEcR and RXR are expressed from a dicistronic message transcribed from the CMV promoter. This mode of expression was achieved by placing the internal ribosome entry site (IRES) between the VgEcR and RXR open reading frames (ORF). This construction allows efficient, high-level internal (cap-independent) initiation of translation of ORFs positioned downstream in an appropriate context. The pERV3 vector also contains a neomycin-resistance gene so that mammalian cell transfectants can be selected with the antibiotic G418.