SP6 RNA Polymerase
SDS-polyacrylamide gel electrophoresis analysis of the SP6 RNA Polymerase assures 99% purity.
Unit definition: One unit will catalyze the incorporation of 1 nmole of nucleoside triphosphate into an acid insoluble form in 60 minutes at 37°C.
Reaction Conditions: 40 mM Tris-HCl (pH 8.0), 8 mM MgCl2, 50 mM NaCl, 2 mM spermidine, 30 mM DTT, 400 µM of each rNTP, 1 µg DNA template and 20 units enzyme in a 25-µl volume. Incubate 60 minutes at 37°C.