Cary 3500 Multicell UV-Vis Spectrophotometer
Cary 3500 Multicell UV-Vis Spectrophotometer
The Cary 3500 comes complete with Cary UV Workstation software—a next-generation analytical platform with streamlined methods for wavelength reads, scanning, concentration, kinetics, and temperature measurements. When configured with Agilent OpenLab data management software, Cary UV Workstation data is managed, protected, and stored in a local or central secure database.
- UV-Vis & UV-Vis-NIR Instruments
Product Details
- Measure samples at four temperatures across eight cuvette positions in a single experiment and collect data from each cuvette simultaneously.
- Control the temperature of your samples accurately and quickly from -5 to 110 °C without water, noise, or messy cables using a unique and fully integrated air-cooled Peltier system.
- Control UV-Vis thermal ramp measurements at up to 40 °C/min using highly accurate, solid-state digital Cary temperature probes that control experimental temperature from inside the cuvette.
- Ensure analytical accuracy for small-volume measurements with a highly-collimated and uniform beam of less than 1.5 mm width, coupled with permanent optical alignment of the stationary cell holders.
- Reduce data processing time and calculation errors with more than 50 built-in and customized calculations that can be saved and applied automatically to any method used routinely.
- Achieve research grade photometric performance, stability, and range with a double beam spectrophotometer featuring leading optical technologies.
- Be up and running in minutes by following the intuitive video tutorials in Cary UV Workstations Help and Learning Center.
- Meet data integrity standards—provides the technical controls to securely acquire, process, report and store data in laboratories that must follow the compliance guidelines of FDA 21 CFR Part 11, EU Annex 11, GAMP5, as well as ISO/IEC 17025 and EPAs 40 CFR Part 160.
- Store and manage your Cary 3500 Multicell UV-Vis spectrophotometer data in a local secured storage on the workstation PC, or in a central storage location.
- Deploy the OpenLab software suite and get optional secure database storage for electronic records, complete administration for users and access privileges, software licensing, and security settings—e-signature workflow and enhanced audit trail review capabilities.
- Choose sustainability: The Cary 3500 Multicell UV-Vis received My Green Labs ACT (Accountability, Consistency, Transparency) label after independent audit for its environmental impact throughout the product lifecycle.
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Xenon Flash Lamps for UV-Vis & Fluorescence Spectrophotometers
For more than 35 years, Agilent has been the pioneer in xenon flash lamp technology for UV-Vis and fluorescence instrumentation. Agilent provides innovative workflow solutions using xenon lamps, including our 10-year lamp warranty, on Cary 60 UV-Vis, Cary 3500 UV-Vis, and Cary Eclipse Fluorescence spectrophotometers.
Learn MoreUV-Vis Analysis of Biomolecules
Analyzing proteins, oligos, monoclonal antibodies, and other biomolecules? UV-Vis spectroscopy is a key part of any bioanalytical toolkit, with many features and benefits that accelerate workflows involving biomolecules. Read our guide and customer case studies to learn more.
Learn MoreDouble Beam Spectrophotometers
Double beam spectrophotometers are fast and accurate, using two beams of light—a sample beam and a reference beam—to correct instrument fluctuations in real-time.
Learn More- Key Literature
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Cary 3500 UV-Vis Brochure
Learn about the Agilent Cary 3500 UV-Vis spectrophotometer
- Brochures
- English
- 04 Sep 2024
- 4.87 MB
Amplify Confidence in Your Data Integrity
How to get the functionality you need for compliance with 21 CFR Part 11 or EU Annex 11 when using the Agilent Cary 3500 UV-Vis instrument.
- Flyers
- English
- 29 Aug 2022
- 749.43 KB
Evaluating an Innovative Analytical ID Testing Strategy for Oligonucleotides
This case study with Vetter Pharma describes how the Agilent Cary 3500 is being used for oligonucleotide identification tests.
- Application Notes
- English
- 26 Feb 2023
- 373.66 KB
- Application Notes
- Brochures
- Catalogs
- Flyers
- Posters
- Primers
- Technical Overviews
- White Papers
- FAQs
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UV-Vis Spectroscopy & Spectrophotometer FAQs
An introduction to the basics of UV-Vis spectroscopy and UV-Vis spectrophotometers, with FAQs on the fundamentals of UV-Vis, how UV-Vis spectrophotometers work, UV-Vis analysis, applications, accessories, and measurement techniques.
- Instruction Sheets
- Site Preparation Checklists
- User Manuals
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Efficient Phase Diagram Analysis of the Ouzo Effect with Cary 3500 UV-Vis Spectroscopy
This study highlights the Cary 3500 UV-Vis spectrophotometer’s ability to rapidly establish a solubility phase diagram for anethole in pastis liqueur. The system completed the phase diagram quickly thanks to parallel temperature ramping experiments (using a Peltier-controlled Multicell module) and independent stirrers ensuring uniform temperature. The Cary 3500 UV-Vis proves to be a powerful tool for analyzing complex samples and understanding the ouzo effect.
- 16 Sep 2024
Explore the Dynamic Briggs-Rauscher Reaction with the Agilent Cary 3500 UV-Vis Spectrophotometer
Our advanced system allows simultaneous analysis at four temperatures, ensuring precise control and swift data capture—vital for observing the rapid color transitions that occur within milliseconds. This application note unveils the capabilities of our spectrophotometer in deciphering complex reaction kinetics through meticulous temperature regulation and cuvette stirring.
- 29 Aug 2024
Agilent Releases Cary UV Workstation 1.5—A Powerful and Flexible Analytical Platform for UV-Vis
The latest Cary UV Workstation software revision comes with new features and enhancements, including compatibility with the new OpenLab 2.8—helping organizations deploy Cary 3500 UV-Vis instruments in distributed environments. Also included are potassium dichromate tests for absorbance and linearity validation. This software version supports Windows 11 21H2, and ECM 3.6.5. The Cary 3500 UV-Vis spectrophotometer series with the Cary UV Workstation software will help meet the requirements of the United States Pharmacopeia USP <857>, European Pharmacopoeia (Ph. Eur. Chapter 2.2.25), and the Japanese Pharmacopeia (JP XVIII) for regulatory peace of mind.
- 11 Jul 2024
Read reviews on the Cary 3500 UV-Vis Spectrophotometer
Explore customer reviews posted on SelectScience, or write your own review.
Thermal Melts Time Saving Calculator
See how much time the Cary 3500 UV-Vis can save your lab compared to your current thermal melt workflows.
UV-Vis Spectroscopy Learning Tools
Get your Basics of UV-Vis Spectrophotometry handbook, and a pack of four undergraduate teaching lab experiments.
Multicell UV-Vis Spectrometer Applications
Discover unique multicell UV-Vis experiment capabilities—for routine analysis and challenging measurements.
Molecular Spectroscopy Pharma & Biopharma Solutions Guide
Learn about products & applications in pharma & biopharma using UV-Vis, FTIR, fluorescence, SORS & TRS technology.
Alexandra Heussner, PhD
Laboratory Manager, Analytical Science Laboratory (ASL), Vetter Pharma-Fertigung GmbH & Co. KG, Ravensburg, Germany
There is a trend towards using melting temperature (Tm) in combination with intact mass measurement, combining sequence and mass to achieve unequivocal identification. Though different analytical techniques have been thoroughly reviewed, this Tm-based strategy has been discussed at expert conferences and is supported by many oligonucleotide-developing and -producing pharmaceutical companies. For this approach, the Cary 3500 UV‑Vis provides state-of-the-art technology. Temperature can be accurately set with the integrated Peltier element and controlled with the in-cuvette probes. The design of the multizone instrument with four cuvette pairs allows measuring samples and references in paral
View MoreCarlos Santos-Martin
PhD Candidate, La Trobe University, Australia
Carlos discusses his work on urinary tract infections, including multicell use of the Cary 3500 UV-Vis.
View MoreBenjamin Tadgell, Eser M. Akinoglu
ARC Centre of Excellence in Exciton Science School of Chemistry, University of Melbourne
We are impressed with the fast scan, user friendly modular design and high throughput of Agilent Cary 3500 UV-Vis. In particular, the great temperature ramping rate and sensitivity makes our work easier. Our project uses UV-Vis to research how temperature affects the scattering properties of gold nanoparticles coated in PNIPAM polymer. As temperature increased over the 30 – 40 °C range, the polymer collapses and the particles aggregate; we are interested in understanding the dynamics of these processes. Wavelength scans at different temperatures, and thermal gradient scans are used to partially characterize these processes. We also do these measurements over a range of pH, salt concentrat
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