![MYC IQFISH Break-Apart Probe MYC IQFISH Break-Apart Probe](/cs/publishingimages/myc_probe_map-thumbnail.jpg)
MYC IQFISH Break-Apart Probe
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Break apart probes consist of two child probes (3 and 5 probes), designed to hybridize on opposite sides of the translocation break point for a given gene, each labeled in a different color (Cy3 and FITC). These probes generate signals in normal cells that are closely matched in size and co-localized (2 fusion signals). Following a translocation, the signals are 'broken apart' and no longer co-localize (for example: 1 orange-red, 1 green, 1 fusion).
MYC IQFISH Break-Apart Probe and corresponding protocol were developed using formalin-fixed, paraffin-embedded, lymph node tissue sections.
Child Probe 5' RD, Chr8
Start 127977211, Stop 128481671
Child Probe 3' GR, Chr8
Start 130342021, Stop 130739576