Mesothelioma

Wilms' Tumor 1 Protein (Autostainer Link 48)

Clone 6F-H2

WT1 is a gene involved in the induction of Wilms' tumor, a pediatric renal malignancy. Wilms' tumor 1 protein regulates transcription of other genes and can function both as a transcriptional activator and repressor. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids. Results aid in the classification of Wilms' tumors, malignant mesotheliomas and acute leukemias.

Monoclonal Mouse Anti-Human

Features

Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.

Specifications


Application	Formalin HIER
Clone	6F-H2
Code Number	IR055
Immunogen	Truncated human WT1 protein corresponding to N-terminal amino acids 1-181 (1,2). See package insert for reference(s).
Isotype	IgG1, kappa
Reagent Provided	Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN₃.
Solutions	Autostainer Link 48
Species	Mouse Anti-Human
Specificity	Alternate splicing of the WT1 transcript gene results in four distinct mRNA species which are present in different amounts. The predicted molecular weight of the WT1 protein ranges from 46 to 49 kDa depending on the mRNA species however, the WT1 proteins derived from cells migrate at 50 to 55 kDa in SDS-PAGE (4). This antibody recognizes an epitope found in the amino terminal 84 encoded amino acids of WT1. It reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2, frequently found in subsets of sporadic Wilms' tumors. In immunoprecipitation assays, the antibody has been shown to recognize full-length and in vitro translated WT1. It also detects full-length denatured WT1 (55 kDa) in Western blots of WT1 baculovirus-infected cell lysates (1). See package insert for reference(s).

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Wilms' Tumor 1 Protein (Autostainer Link 48)

Product Details

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