Tyrosinase Antibody (Concentrate)

Features

Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years’ experience ensuring quality and innovation in antibodies and reagents

Specifications


Application	Formalin HIER
Clone	T311
Code Number	M3623
Immunogen	Purified recombinant unglycosylated tyrosinase protein consisting of 452 amino acids of the 511 amino acid mature human tyrosinase protein (1). See package insert for reference(s).
Isotype	IgG2a, kappa
Reagent Provided	Monoclonal mouse antibody provided in liquid form as tissue culture supernatant in 0.05 mol/L Tris-HCl, pH 7.2 and 0.015 mol/L sodium azide. This product contains stabilizing protein.
Solutions	Concentrate
Species	Mouse Anti-Human
Specificity	Immunoblotting was performed using L-cells transfected with the tyrosinase gene and on four cell lines that express tyrosinase mRNA: SK-MEL-13, SK-MEL-19, SK-MEL-30 and SK-MEL-37 (1). Consistent with previous reports, a cluster of proteins ranging from 70-80 kDa was recognized by clone T311 in immunoblots of all five cell lines (1). An additional 55 kDa band was stained in blots from cell lines that are strongly reactive (SK-MEL-19) (1). Furthermore, immunoblots of untransfected L-cells and three cell lines that do not express tyrosinase mRNA (MZ2-MEL3.1, SK-MEL-187 and MZ2-MEL2.2) were negative with clone T311 (1). L-cells transfected with the tyrosinase-related protein 1, TRP-1(gp75), were also unreactive with clone T311 in immunofluorescence and immunoblotting assays, indicating this antibody does not cross-react with the melanosome-associated protein, TRP-1(gp75) (1). See package insert for reference(s).

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Literature

Technical Overviews

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.

Technical Overviews
English
30 Nov 2021
27.05 MB
PDF

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Tyrosinase (Concentrate)

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Immunohistochemical Staining Methods - Education Guide, Sixth Edition

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