The antigen has been isolated from primary granulocytes (1). The antibody reacts with neutrophil granulocytes and monocytes in blood and with precursors of granulocytes in the bone marrow. The antibody is useful as an aid for classification of neoplastic tissue, i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma (2).
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Product Details
Features
Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years experience ensuring quality and innovation in antibodies and reagents
Specifications
Application
Formalin
HIER
Clone
Polyclonal
Code Number
A0398
Immunogen
Myeloperoxidase isolated from human polymorphonuclear leucocytes.
Reagent Provided
Purified immunoglobulin fraction of rabbit antiserum provided in liquid form. In 0.1 mol/L NaCl, 15 mmol/L NaN3.
Solutions
Concentrate
Species
Rabbit Anti-Human
Specificity
The antibody reacts with human myeloperoxidase. In crossed immunoelectrophoresis using 12.5 µL antibody per cm2 gel area against 2 µL myeloperoxidase only one precipitate corresponding to myeloperoxidase appears. Staining: Specific staining for peroxidase. With 2 µL of human plasma, no precipitate appears. Staining: Coomassie Brilliant Blue.
In formalin-fixed, paraffin-embedded human tonsil, and when using a sensitive streptavidin-biotin-based visualization system, the antibody as expected labels only rare neutrophil granulocytes.
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.
1. Matheson NR, Wong PS, Travis J. Isolation and properties of human neutrophil myeloperoxidase. Biochem 1981,20:325-30. 2. Pinkus GS, Pinkus JI. Myeloperoxidase: a specific marker for myeloid cells in paraffin sections. Mod Pathol 1991,4:733-41.