Ki-67 Antigen (Dako Omnis)

Clone MIB-1

With more than 4000 literature citations, the MIB-1 antibody has now been established as an important monoclonal mouse antibody for the demonstration of the Ki-67 antigen in formalin-fixed, paraffin-embedded specimens. The Ki-67 antigen is a large nuclear protein (345, 395 kDa) preferentially expressed during all active phases of the cell cycle (G₁, S, G₂ and M-phases), but absent in resting cells (G₀-phase). The antibody is a useful aid for classification of a variety of tumors.

Monoclonal Mouse Anti-Human

Features

Optimized staining performance of both high and low-expression structures
Dako Omnis and the dynamic gap staining technology provides a consistent, uniform staining with excellent morphology
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Dako Omnis
The staining performance of all FLEX RTU antibodies has been defined, tested and approved through collaboration with leading, international pathology experts.

Specifications


Application	Formalin HIER
Clone	MIB-1
Code Number	GA626
Immunogen	Human recombinant peptide corresponding to a 1002 bp Ki-67 cDNA fragment (6). See package insert for reference(s).
Isotype	IgG1, kappa
Reagent Provided	Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Solutions	Dako Omnis
Species	Mouse Anti-Human
Specificity	In Western blotting of lysates of the multiple myeloma cell line, IM-9, the MIB-1 antibody labels bands of 345 and 395 kDa, identical to the bands labeled by the original Ki-67 antibody. Furthermore, Western blotting and competitive binding experiments clearly demonstrate that MIB-1, like the original Ki-67 antibody, reacts with an epitope encoded by a 66 bp repetitive element in the Ki-67 gene. In immunohistochemistry, the MIB-1 and the Ki-67 antibodies provide identical staining patterns on serial tonsillar frozen sections (6). The MIB-1 antibody recognizes native Ki-67 antigen and recombinant fragments of the Ki-67 molecule (6). See package insert for reference(s).

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Featured References

Product Description References: 1. Gerdes J, Becker MH, Key G, Cattoretti G. Immunohistological detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J Pathol 1992,168:85-6.
2. Cattoretti G, Becker MH, Key G, Duchrow M, Schlüter C, Galle J, et al. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol 1992,168:357-63.
3. Scholzen T, Gerdes J. The Ki-67 protein: from the known and the unknown [review]. J Cell Physiol 2000,182:311-22.

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Ki-67 Antigen (Dako Omnis)

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