Ki-67 Antigen Antibody (Concentrate)

Features

Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years’ experience ensuring quality and innovation in antibodies and reagents

Specifications


Application	Formalin HIER
Clone	MIB-1
Code Number	M7240
Immunogen	Human recombinant peptide corresponding to a 1002 bp Ki-67 cDNA fragment (6). See package insert for reference(s).
Isotype	IgG1, kappa
Reagent Provided	Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, 1% bovine serum albumin, pH 7.2, and containing 15 mmol/L NaN₃.
Solutions	Concentrate
Species	Mouse Anti-Human
Specificity	In Western blotting of lysates of the multiple myeloma cell line, IM-9, the MIB-1 antibody labels bands of 345 and 395 kDa, identical to the bands labeled by the original Ki-67 antibody. Furthermore, Western blotting and competitive binding experiments clearly demonstrate that MIB-1, like the original Ki-67 antibody, reacts with an epitope encoded by a 66 bp repetitive element in the Ki-67 gene. In IHC the MIB-1 and the Ki-67 antibodies provide identical staining patterns on serial tonsillar frozen sections (6). The MIB-1 antibody recognizes native Ki-67 antigen and recombinant fragments of the Ki-67 molecule (6).

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Literature

Technical Overviews

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.

Technical Overviews
English
30 Nov 2021
27.05 MB
PDF

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Featured References

Product Description References: 1. Gerdes J, Becker MH, Key G, Cattoretti G. Immunohistological detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J Pathol 1992,168:85-6.
2. Cattoretti G, Becker MH, Key G, Duchrow M, Schlüter C, Galle J, et al. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol 1992,168:357-63.
3. Scholzen T, Gerdes J. The Ki-67 protein: from the known and the unknown [review]. J Cell Physiol 2000,182:311-22.

Featured

Ki-67 Antigen (Concentrate)

Product Details

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

View supported Instructions For Use documents

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