Breast tissue with positive estrogen receptor expression
Estrogen Receptor α (Autostainer Link 48)
Clone EP1
This monoclonal rabbit antibody reacts with human estrogen receptor α (ER α). Estrogens have been found to be preferentially concentrated in the estrogen target organs of animals and in human breast cancers, and it is well documented that the mitogenic effects of estrogen are mediated by ER. Historical studies have shown that ER status is correlated with untreated outcome and with response to anti-hormonal therapy, e.g. tamoxifen (1). The antibody can used in the semi-quantitative detection of human estrogen receptor in tissue sections of human breast cancer by immunohistochemistry for assessment of estrogen receptor status in human breast carcinomas.
This product is not available for purchase by the general public.
Product Details
Features
Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.
Specifications
Application
Formalin
HIER
Clone
EP1
Code Number
IR084
Immunogen
Recombinant protein of ER α amino acids 1-300.
Isotype
Rabbit IgG
Reagent Provided
Ready-to-use monoclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Solutions
Autostainer Link 48
Species
Rabbit Anti-Human
Specificity
In Western blotting of MCF7 cells lysates the antibody labels a major band of approximately 67 kDa, corresponding to the expected molecular weight of ER α. No cross-reactivity was seen with ER β.