This monoclonal rabbit antibody reacts with cyclin D1, a 36 kDa protein encoded by the CCND1 (bcl-1) gene located on chromosome 11q13. Cyclin D1 is part of the cell cycle regulation and oncogenic transformation in mammalian cells. The cyclin-dependent kinases, CDK4 and CDK6, are associated with and activated by cyclin D1 thereby promoting G1 phase progression by retinoblastoma protein phosphorylating along with related proteins. Antibodies to cyclin D1 are a useful aid for classification of mantle cell lymphomas in the context of lymphoid tumors (1-2).
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Product Details
Features
Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years experience ensuring quality and innovation in antibodies and reagents
Specifications
Application
Formalin
HIER
Clone
EP12
Code Number
M3642
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human cyclin D1.
Isotype
Rabbit IgG
Reagent Provided
Monoclonal rabbit antibody provided in liquid form as affinity-isolated antibody in 0.02 mol/L phosphate buffer, pH 6.9-7.2, and containing 0.1 mol/L NaCl, and 0.015 mol/L sodium azide. This product contains stabilizing protein.
Solutions
Concentrate
Species
Rabbit Anti-Human
Specificity
In Western blotting of MCF7 cells lysates the antibody labels a major band of approximately 36 kDa, corresponding to the expected molecular weight of cyclin D1.
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.
1. Donnellan R, Chetty R. Cyclin D1 and human neoplasia. J Clin Pathol: Mol Pathol 1998,51:1-7. 2. Falini B, Martelli MP, Tiacci E, Ascani S, Thiede C, Pileri SA. Immunohistochemical surrogates for genetic alterations of CCND1, PML, ALK, and NPM1 genes in lymphomas and acute myeloid leukemia. Best Practice & Research Clin Haematol 2010:23:417-31.