CD79α Antibody (Concentrate)

Features

Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years’ experience ensuring quality and innovation in antibodies and reagents

Specifications


Application	Formalin HIER
Clone	JCB117
Code Number	M7050
Immunogen	Recombinant protein containing part of the extracellular portion of the human CD79α glycoprotein (1). See package insert for reference(s).
Isotype	IgG1, kappa
Reagent Provided	Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN₃.
Solutions	Concentrate
Species	Mouse Anti-Human
Specificity	The antibody was clustered as anti-CD79α at the Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens held in Kobe in 1996 (4). In Western blotting of Ramos B-cell lysate, the antibody labels a band corresponding to CD79α under reducing conditions, and a band corresponding to CD79αß under non-reducing conditions (1). SDS-PAGE analysis of immunoprecipitates formed between the antibody and ¹²⁵I-labeled, mildly denatured, and reduced lysate of Ramos B cells, shows weak reaction with a 43 kDa polypeptide corresponding to CD79α. The antibody was tested by flow cytometry against normal peripheral B-cell lines, and against mature and immature B-cell lines (Ramos, Raji, and NALM-1), and was consistently negative, indicating that the epitope recognized is not present, or is not accessible, on living cells. However, the antibody specifically labels B cells in paraffin-embedded tissue sections (4). See package insert for reference(s).

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Literature

Technical Overviews

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.

Technical Overviews
English
30 Nov 2021
27.05 MB
PDF

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Featured References

Product Description References: 1. Mason DY, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, et al. CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood 1995,86:1453-9.
2. Pilozzi E, Pulford K, Jones M, Muller-Hermelink HK, Falini B, Ralfkiaer E, et al. Co-expression of CD79a (JCB117) and CD3 by lymphoblastic lymphoma. J Pathol 1998,186:140-3.
3. Chu PG, Arber DA. CD79: a review. Appl Immunohistochem Mol Morphol 2001,9:97-106.

Featured

CD79α (Concentrate)

Product Details

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

View supported Instructions For Use documents

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