CD34 is a single-chain transmembrane protein of approximately 116 kDa, expressed on immature hematopoietic stem/progenitor cells, capillary endothelial cells, embryonic fibroblasts and rare glial cells in nervous tissue. CD34 is a stage-specific, rather than a lineage-specific, leucocyte differentiation antigen. The antibody is a useful aid for classificaction of vascular and lymphatic tumors and for the subclassification of leukemias.
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Product Details
Features
Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years experience ensuring quality and innovation in antibodies and reagents
Specifications
Application
Formalin
Enzyme/HIER
Clone
QBEnd 10
Code Number
M7165
Immunogen
Endothelial cell membranes obtained as vesicles from human placenta (2, 3).
Isotype
IgG1, kappa
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN3.
Solutions
Concentrate
Species
Mouse Anti-Human
Specificity
Anti-CD34, QBEnd 10, was included in the Fourth and Fifth International Workshops and Conferences on Human Leucocyte Differentiation Antigens (Vienna 1989, Boston 1993), and studies by different laboratories confirmed its reactivity with the CD34 antigen (6, 8) and the class II epitope (8). In formalin-fixed, paraffin embedded tonsil sections the antibody labels endothelial cells. In flow cytometry the antibody labels KG-1a cells (a primitive myeloid leukaemia cell line, known to be CD34+). See package insert for reference(s).
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.