CD31, Endothelial Cell Antibody (Dako Omnis)

Features

Optimized staining performance of both high and low-expression structures
Dako Omnis and the dynamic gap staining technology provides a consistent, uniform staining with excellent morphology
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Dako Omnis
The staining performance of all FLEX RTU antibodies has been defined, tested and approved through collaboration with leading, international pathology experts.

Specifications


Application	Formalin HIER
Clone	JC70A
Code Number	GA610
Immunogen	Cell membrane preparation from the spleen of a patient with hairy cell leukemia (1). See package insert for reference(s).
Isotype	IgG1, kappa
Reagent Provided	Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Solutions	Dako Omnis
Species	Mouse Anti-Human
Specificity	Anti-Human CD31, Endothelial Cell, clone JC70A, was clustered as anti-CD31 at the Fifth International Workshop and Conference on Human Leucocyte Differentiation Antigens (4). The epitope recognized was found to be within the extracellular domain 1 (3). In Western blotting of membrane preparations from a spleen rich in the antigen or from normal platelets, the antibody labels bands of respectively 100 kDa and 130 kDa, the latter corresponding to classic CD31. The smaller band of 100 kDa observed with the splenic preparation may be due to proteolytic breakdown or to variations in glycosylation (1). See package insert for reference(s).

Literature

Catalogs

The Atlas of Controls illustrates the staining performance of each antibody in the FLEX Ready-to-Use system.

Technical Overviews

Support

Featured References

Product Description References: 1. Parums DV, Cordell JL, Micklem K, Heryet AR, Gatter KC, Mason DY. JC70: a monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections. J Clin Pathol 1990,43:572-7.
2. Kuzu I, Bicknell R, Harris AL, Jones M, Gatter KC, Mason DY. Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours. J Clin Pathol 1992,45:143-8.
3. Ohsawa M, Naka N, Tomita Y, Kawamori D, Kanno H, Aozasa K. Use of immunohistochemical procedures in diagnosing angiosarcoma. Evaluation of 98 cases. Cancer 1995,75:2867-74.

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CD31, Endothelial Cell (Dako Omnis)