Synthetic peptide from the intracellular part of the e-chain of human CD3 was coupled to bovine thyroglobulin and used for immunization. The antibody is a pan-T cell marker for identification of T cells. It is well-suited for labeling reactive T cells in tissue with lymphoid infiltrates, and for classification of T-cell neoplasms. The antibody shows a stronger labeling intensity than corresponding monoclonal antibodies to CD3, and should, generally, be preferred on formalin-fixed, paraffin-embedded tissue sections.
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Product Details
Features
Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years experience ensuring quality and innovation in antibodies and reagents
Specifications
Application
Frozen
Formalin
HIER
Clone
Polyclonal
Code Number
A0452
Immunogen
Synthetic peptide comprising amino acids 156-168 from the cytoplasmic part of the human CD3e-chain coupled to thyroglobulin (1). See package insert for reference(s).
Reagent Provided
Affinity-isolated rabbit anti-human CD3 provided in liquid form. In 0.05 mol/L Tris-HCl, 0.1 mol/L NaCl, 15 mmol/L NaN3. The affinity isolation has been performed using immobilized CD3 peptide.
Solutions
Concentrate
Species
Rabbit Anti-Human
Specificity
In Western blotting, the antibody detects bands of the expected molecular weights for CD3 antigens (2). The antibody recognizes CD3e in both a T-cell line (Jurkat) and a natural killer cell line (NK11), but does not react with lysates prepared from several B-cell lines (Raji, Ramos and JY), a myeloid cell line (U937) or a colon carcinoma cell line (Colo-205) (9).
In immunoprecipitation from Nonidet P40 lysates of surface-iodated T lymphoblasts, the antibody precipitates the γ (26 kDa), δ (21 kDa) and e (19 kDa) chain of the CD3 molecule, similar to the precipitation pattern seen using the well-characterized monoclonal mouse anti-human CD3, clone UCHT1 (1).
In ELISA, the antibody labels the CD3 peptide used as immunogen.
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