B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma

CD20cy (Dako Omnis)

Clone L26

CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B cells, but is lost following differentiation into plasma cells. In resting B cells, CD20 appears in a 33 kDa non-phosphorylated form. After mitogen stimulation, CD20 becomes heavily phosphorylated (35-37 kDa isoforms), and it is a dominant phosphoprotein in activated B cells. The antibody reacts with an intracytoplasmic epitope localized on the CD20 antigen and labels cells of the B-cell lineage. It is a useful aid for classification of neoplasms of B-cell derivation.

Monoclonal Mouse Anti-Human

Features

Optimized staining performance of both high and low-expression structures
Dako Omnis and the dynamic gap staining technology provides a consistent, uniform staining with excellent morphology
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Dako Omnis
The staining performance of all FLEX RTU antibodies has been defined, tested and approved through collaboration with leading, international pathology experts.

Specifications


Application	Formalin HIER
Clone	L26
Code Number	GA604
Immunogen	Human tonsil B cells (4). See package insert for reference(s).
Isotype	IgG2a, kappa
Reagent Provided	Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Solutions	Dako Omnis
Species	Mouse Anti-Human
Specificity	Anti-Human CD20cy, clone L26, was clustered as anti-CD20 at the Fifth International Workshop and Conference on Human Leucocyte Differentiation Antigens (2). SDS-PAGE analysis of immunoprecipitates formed between ¹²⁵I-labeled tonsil cell lysate and the antibody shows reaction primarily with 30 kDa and 33 kDa polypeptides (4). Studies using COS-1 cells transfected with cDNA encoding the CD20 molecule, indicate that the antibody labels an intracytoplasmic epitope localized on the CD20 molecule (5). See package insert for reference(s).

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CD20cy (Dako Omnis)

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