CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B cells, but is lost following differentiation into plasma cells. In resting B cells, CD20 appears in a 33 kDa non-phosphorylated form. After mitogen stimulation, CD20 becomes heavily phosphorylated (35-37 kDa isoforms), and it is a dominant phosphoprotein in activated B cells. The antibody reacts with an intracytoplasmic epitope localized on the CD20 antigen and labels cells of the B-cell lineage. It is a useful aid for classification of neoplasms of B-cell derivation.
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Product Details
Features
Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years experience ensuring quality and innovation in antibodies and reagents
Specifications
Application
Frozen
Formalin
HIER
Clone
L26
Code Number
M0755
Immunogen
Human tonsil B cells (4). See package insert for reference(s).
Isotype
IgG2a, kappa
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN3.
Solutions
Concentrate
Species
Mouse Anti-Human
Specificity
The antibody was clustered as anti-CD20 at the Fifth International Workshop and Conference on Human Leucocyte Differentiation Antigens held in Boston 1993 (2). SDS-PAGE analysis of immunoprecipitates formed between 125I-labeled tonsil cell lysate and the antibody shows reaction primarily with 30 kDa and 33 kDa polypeptides (4). Studies using COS-1 cells transfected with cDNA encoding the CD20 molecule, indicate that the antibody labels an intracytoplasmic epitope localized on the CD20 molecule (5). See package insert for reference(s).
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.