Reacts with a carbohydrate antigen, termed Lewis X (Lex), X hapten or CD15 antigen, expressed on Reed-Sternberg cells and various other cell types including myeloid cells and epithelial cells. Results aid in the classification of acute myeloid leukemia and chronic myelogenous leukemia, as well as carcinomas derived from various organs (1). The antibody is of value in the identification of Reed-Sternberg cells for classification of Hodgkin's lymphoma.
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Product Details
Features
Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years experience ensuring quality and innovation in antibodies and reagents
Specifications
Application
Formalin
HIER
Clone
Carb-3
Code Number
M3631
Immunogen
Immuno-affinity purified X-Hapten oligopeptide fragment from myelomonocytic leukemia cells.
Isotype
IgM
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 0.015 mol/L sodium azide. This product contains stabilizing protein.
Solutions
Concentrate
Species
Mouse Anti-Human
Specificity
In Western blotting of HL60 cell lysates the antibody labels a major band of ~220 kDa corresponding to the expected molecular weight of CD15 (7). See package insert for reference(s).
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.