Recognizes a 382-amino-acid protein, alpha-methylacyl-CoA racemase (AMACR), that was identified by cDNA library subtraction in conjunction with high throughput microarray screening of prostate adenocarcinomas. AMACR, also known as P504S, is an enzyme that is involved in bile acid biosynthesis and β-oxidation of branched-chain fatty acids. Results aid in the classification of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma (1). AMACR is present at low or undetectable levels in glandular epithelial cells of normal and benign hyperplastic prostates.
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Product Details
Features
Optimized staining performance of both high and low-expression structures
Dako Omnis and the dynamic gap staining technology provides a consistent, uniform staining with excellent morphology
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Dako Omnis
The staining performance of all FLEX RTU antibodies has been defined, tested and approved through collaboration with leading, international pathology experts.
Specifications
Application
Formalin
HIER
Clone
13H4
Code Number
GA060
Immunogen
Full length recombinant AMACR (9). See package insert for reference(s).
Isotype
Rabbit IgG
Reagent Provided
Ready-to-use monoclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
Solutions
Dako Omnis
Species
Rabbit Anti-Human
Specificity
The specificity of monoclonal rabbit anti-AMACR was evaluated by immunocytochemistry and Western blot analysis. Anti-AMACR positively bound formalin-fixed HEK293 cells which overexpressed AMACR, but was unreactive with cells transfected with an empty plasmid. In Western blots of lysates from primary prostate carcinoma samples, monoclonal rabbit anti-AMACR identified a 54 kDa protein corresponding to the expected molecular weight of AMACR (2). See package insert for reference(s).
1. Luo J, Zha S, Gage WR, Dunn TA, Hicks JL, Bennett CJ, et al. Alpha-methylacyl-CoA racemase: a new molecular marker for prostate cancer. Cancer Res 2002,62:220-6.