RNA Spike-In Kits
RUO
Agilents RNA spike-In kit lets researchers efficiently monitor their microarray workflow for linearity, sensitivity, and accuracy. Each spike mix in these one-color and two-color RNA spike-in kits contains 10 in vitro synthesized, polyadenylated transcripts in predetermined ratios.
When the transcripts are hybridized onto Agilent microarray control probes, the data needed to track performance and assure confidence is readily accessible.
When the transcripts are hybridized onto Agilent microarray control probes, the data needed to track performance and assure confidence is readily accessible.
For Research Use Only. Not for use in diagnostic procedures.
- Gene Expression Microarray Kits & Reagents
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This product is not available for purchase by the general public.
Product Details
Features
- Each transcript was constructed by the cloning of a unique 55-mer sequence into the human adenovirus type 6 E1A 13S gene
- Allows the user to monitor microarray workflow from sample amplification and labeling to microarray processing
- Spike-In transcripts specifically anneal only to complementary control probes on Agilent's microarrays, with minimal self- or cross-hybridization
- Analyze Spike-Ins using Agilent Feature Extraction software
- Kit should be stored initially at -80°C for up to one year from date of result. Diluted spike mixes can be stored at -20°C for up 2 months
Literature
- Application Notes
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ERCC Spike-In Control Transcripts Provides Confidence in Agilent Microarray and RNA-Seq Gene Expression Data
Data generated by the Sequencing Quality Control Consortium (SEQC) has shown that RNA-Seq and microarray-based gene expression models are comparable in clinical endpoint prediction performance.
- Application Notes
- English
- 21 Feb 2020
- 6.21 MB
Ultra-low Input Total RNA Gene Expression Analysis Using the Agilent SurePrint G3 Human Gene Expression Microarray
In this study, we modified the existing gene expression microarray protocol to allow processing of samples starting from 250 pg of total RNA.
- Application Notes
- English
- 20 Nov 2019
- 1.72 MB
- Brochures
Support
- User Manuals
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Agilent Two Color RNA Spike-in Kit Protocol
This is a protocol sheet for the Agilent Two Color RNA Spike-In Kit. It details kit contents, storage, protocol, and representative data obtained using the included protocol.
- User Manuals
- English
- 04 May 2016
- 735.22 KB
One-Color Microarray-Based Gene Expression Analysis v.6.9.1
One-Color Microarray-Based Gene Expression Analysis 6.9.1
- User Manuals
- English
- 31 Mar 2016
- 5.07 MB
Agilent One Color RNA Spike-in Kit Protocol
Agilent One Color RNA Spike-in Kit Protocol 2nd Edition
- User Manuals
- English
- 18 Dec 2015
- 166.92 KB
Two-Color Microarray-Based Gene Expression Analysis v.6.9.1
Two-Color Gene Expression Protocol 6.9
- User Manuals
- English
- 17 Dec 2015
- 1.71 MB
Microarray-Based Gene Expression Analysis with Tecan HS Pro Hybridization Supplement A0
Microarray-Based Gene Expression Analysis with Tecan HS Pro Hybridization Supplement A0
- User Manuals
- English
- 08 Sep 2015
- 438.22 KB
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