SureVector Library Cloning Kit
RUO
The library cloning kit uses Agilent’s SureVector chemistry to allow the rapid generation of plasmid libraries from an amplified oligo pool. Included in the kit are Agilent’s linearized pSGLenti vector backbone, competent cells, media, and all buffers and reagents required to clone Agilent’s pooled ready-to-clone or ready-to-amplify libraries.
pSGLenti is directly compatible with Agilent’s ready-to-clone libraries while alternative backbones, supplied separately, can also be used to clone fully custom, ready-to-amplify libraries. Each kit contains sufficient amounts of the above materials to generate four plasmid libraries of 60,000 guides.
pSGLenti is directly compatible with Agilent’s ready-to-clone libraries while alternative backbones, supplied separately, can also be used to clone fully custom, ready-to-amplify libraries. Each kit contains sufficient amounts of the above materials to generate four plasmid libraries of 60,000 guides.
For Research Use Only. Not for use in diagnostic procedures.
Product Details
Features
- Plasmid libraries generated optimized to work in mammalian cells with expression driven by a human U6 promoter
- SureVector cloning technology adapted to insert a custom library into the lentiviral plasmid pSGLenti
Featured References
- Publications
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- Braman JC, Sheffield PJ (2019) Seamless assembly of DNA parts into functional devices and higher order multi-device systems. PLoS ONE 14 (6): e0199653. ttps://doi.org/10.1371/journal.pone.0199653 Learn More
- Mullinax R. et al. (2018) A Rapid Combinatorial Approach to Assembling Synthetic Prokaryotic and Eukaryotic Protein Expression Vectors. In: Braman J. (eds) Synthetic Biology. Methods in Molecular Biology, vol 1772. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7795-6_27 Learn More
- Carstens C.P., Felts K.A., Johns S.E. (2018) Construction of CRISPR Libraries for Functional Screening. In: Braman J. (eds) Synthetic Biology. Methods in Molecular Biology, vol 1772. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7795-6_7 Learn More
- Wojnicka, M.; Szczepanska, A.; Kurzynska-Kokorniak (2020), A. Unknown Areas of Activity of Human Ribonuclease Dicer: A Putative Deoxyribonuclease Activity. Molecules, 25, no. 6: 1414. https://doi.org/10.3390/molecules25061414 Learn More
- Gianna Palmieri, Stefania Arciello, Marida Bimonte, Antonietta Carola, Annalisa Tito, Marta Gogliettino, Ennio Cocca, Carmela Fusco, Marco Balestrieri, Maria Gabriella Colucci, Fabio Apone, (2019) The extraordinary resistance to UV radiations of a manganese superoxide dismutase of Deinococcus radiodurans offers promising potentialities in skin care applications, Journal of Biotechnology, Volume 302, Pages 101-111, ISSN 0168-1656, https://doi.org/10.1016/j.jbiotec.2019.07.002 Learn More
