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SureGuide Purified sgRNA
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- CRISPR Chemically Synthesized sgRNA
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Product Details
- Complete de-novo synthesis of fully customizable RNA for use with Cas9 or any other novel enzyme using guides 90 – 120 nucleotides in length
- Purified using a proprietary method developed internally at Agilent
- Stringent LC-MS Quality Control for high reproducibility
- Flexible chemistry with up to 10 modifications (M, MS, S, Deoxy) including our patented 3XMS end-modifications
- Quantities ranging from 100 µg to 20 mg (in 1mg aliquots)
- Easy to use online ordering tool
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- Data Sheets
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SureGuide Chemically Synthesized CRISPR Guide RNA
Datasheet highlighting SureGuide Purified Chemically Synthesized sgRNA in CRISPR gene editing studied
- Data Sheets
- English
- 13 Sep 2022
- 362.63 KB
- Flyers
- Posters
- User Manuals
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SureGuide Single Guide RNAs Product Overview
Storage information, reconstitution instructions, and transfection guidelines for the SureGuide Single Guide RNAs
- User Manuals
- English
- 27 Mar 2022
- 112.39 KB
- Publications
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- Dellinger et al. “Streamlined Process for the Chemical Synthesis of RNA Using 2’-O-Thionocarbamate-Protected Nucleoside Phosphoramidites in the Solid Phase”, J. Am. Chem. Soc. 133, 11540–11556 (2011).
- Ayal Hendel, Rasmus O Bak, Joseph T Clark, Andrew B Kennedy, Daniel E Ryan, Subhadeep Roy, Israel Steinfeld, Benjamin D Lunstad, Robert J Kaiser, Alec B Wilkens, Rosa Bacchetta, Anya Tsalenko, Douglas Dellinger, Laurakay Bruhn & Matthew H Porteus. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nature Biotechnology, volume 33 number 9 (2015); doi:10.1038/nbt.3290
- Daniel E. Ryan, David Taussig, Israel Steinfeld, Smruti M. Phadnis, Benjamin D. Lunstad, Madhurima Singh, Xuan Vuong, Kenji D. Okochi, Ryan McCaffrey, Magdalena Olesiak, Subhadeep Roy, Chong Wing Yung, Bo Curry, Jeffrey R. Sampson, Laurakay Bruhn, and Douglas J. Dellinger. Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs. Nucleic Acids Research, Vol. 46, No. 2 (2018); doi: 10.1093/nar/gkx1199
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Easy online ordering with our simple and easy-to-use CRISPR sgRNA ordering tool
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