SureGuide Purified sgRNA
RUO
Leverages Agilent's unique ability to synthesize high-fidelity custom CRISPR guide RNAs with our new SureGuide Purified single-guide RNAs. SureGuide Purified sgRNAs are fully customizable for unmatched flexibility and include our published, proven, and patented activity enhancing end-modifications. SureGuide Purified sgRNAs are made using a proprietary chemistry and purification method with improved turn-around times alongside stringent quality control for industry leading consistency that gives reliable, reproducible results.
For patent and license information please click here.
For patent and license information please click here.
For Research Use Only. Not for use in diagnostic procedures.
Ready to order your guide RNA? Use our online CRISPR sgRNA Ordering Tool
Product Details
Features
- Complete de-novo synthesis of fully customizable RNA for use with Cas9 or any other novel enzyme using guides 90 – 120 nucleotides in length
- Purified using a proprietary method developed internally at Agilent
- Stringent LC-MS Quality Control for high reproducibility
- Flexible chemistry with up to 10 modifications (M, MS, S, Deoxy) including our patented 3XMS end-modifications
- Quantities ranging from 100 µg to 20 mg (in 1mg aliquots)
- Easy to use online ordering tool
Featured References
- Publications
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- Dellinger et al. “Streamlined Process for the Chemical Synthesis of RNA Using 2’-O-Thionocarbamate-Protected Nucleoside Phosphoramidites in the Solid Phase”, J. Am. Chem. Soc. 133, 11540–11556 (2011).
- Ayal Hendel, Rasmus O Bak, Joseph T Clark, Andrew B Kennedy, Daniel E Ryan, Subhadeep Roy, Israel Steinfeld, Benjamin D Lunstad, Robert J Kaiser, Alec B Wilkens, Rosa Bacchetta, Anya Tsalenko, Douglas Dellinger, Laurakay Bruhn & Matthew H Porteus. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nature Biotechnology, volume 33 number 9 (2015); doi:10.1038/nbt.3290
- Daniel E. Ryan, David Taussig, Israel Steinfeld, Smruti M. Phadnis, Benjamin D. Lunstad, Madhurima Singh, Xuan Vuong, Kenji D. Okochi, Ryan McCaffrey, Magdalena Olesiak, Subhadeep Roy, Chong Wing Yung, Bo Curry, Jeffrey R. Sampson, Laurakay Bruhn, and Douglas J. Dellinger. Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs. Nucleic Acids Research, Vol. 46, No. 2 (2018); doi: 10.1093/nar/gkx1199
