SurePrint G3 CGH & CGH+SNP Microarrays
The Agilent Human CGH and CGH+SNP Microarray offerings are dual-color microarrays containing 60-mer high quality probes. These are high-resolution tools for genome-wide DNA copy number variation profiling, and +SNP option to capture copy-neutral loss of heterozygosity (cnLOH), without amplification or complexity reduction.
For CGH-only arrays, options exist for genome-wide median probe spacing of 43kb, 41kb, 13kb, 5.3kb or 2.1kb (design size 44K, 60K, 180K, 400K and 1M respectively). And all designs have enhanced coverage on known genes, promoters, miRNAs, PAR, and telomeric regions. And the CGH+SNP 400K array is designed with 7.2 KB overall median probe spacing and enhanced coverage on known genes, promoters, miRNAs, PAR and telomeric regions. Each CGH+SNP microarray includes 292,097 (CGH) and 119,091 (SNP) distinct biological features.
For CGH-only arrays, options exist for genome-wide median probe spacing of 43kb, 41kb, 13kb, 5.3kb or 2.1kb (design size 44K, 60K, 180K, 400K and 1M respectively). And all designs have enhanced coverage on known genes, promoters, miRNAs, PAR, and telomeric regions. And the CGH+SNP 400K array is designed with 7.2 KB overall median probe spacing and enhanced coverage on known genes, promoters, miRNAs, PAR and telomeric regions. Each CGH+SNP microarray includes 292,097 (CGH) and 119,091 (SNP) distinct biological features.
For Research Use Only. Not for use in diagnostic procedures.
Product Details
Features
- Agilent provides validated protocols and reagents to process fresh frozen DNA (SureTag DNA Labeling Kit) and degraded or FFPE samples (ULS Labeling Kit)
- Probe design and selection have been carefully optimized and validated for maximal sensitivity and specificity, and each microarray contains internal quality control features
- CGH+SNP 2x400K - 7.2 Kb overall median probe spacing (4.5 Kb in Refseq genes), each microarray has 292,097 (CGH) and 119,091 (SNP) probes annotated against NCBI Build 38 (UCSC GRCh38, Dec. 2013), Design ID/AMADID# 028081
- CGH 2x400K - 5.3 KB overall median probe spacing (4.6 KB in Refseq genes), each microarray contains over 411,056 distinct biological probes annotated against NCBI Build 37 (UCSC hg19, February 2009)
- CGH 1x1M - 2.1 KB overall median probe spacing (1.8 KB in Refseq genes) each microarray contains over 963,029 distinct biological probes annotated against NCBI Build 37 (UCSC hg19, February 2009)
- CGH 8x60K - 41 Kb overall median probe spacing (33 Kb in Refseq genes) each microarray contains over 55,077 distinct biological probes annotated against NCBI Build 38 (UCSC GRCh38, Dec. 2013)
- CGH 4x180K - 13 KB overall median probe spacing (11 KB in Refseq genes) each microarray contains over 170,334 distinct biological probes annotated against NCBI Build 37 (UCSC hg19, February 2009)
- CGH 4x44K - 43 KB overall median probe spacing (24 KB in Refseq genes) each microarray contains over 42,494 distinct biological probes annotated against NCBI Build 36 (UCSC hg18, Mar 2006), Design ID/AMADID# 014950
Featured References
- Publications
-
- Chromosomal microarray analysis of Bulgarian patients with epilepsy and intellectual disability. Peycheva V, Kamenarova K, Ivanova N, Stamatov D, Avdjieva-Tzavella D, Alexandrova I, Zhelyazkova S, Pacheva I, Dimova P, Ivanov I, Litvinenko I, Bozhinova V, Tournev I, Simeonov E, Mitev V, Jordanova A, Kaneva, R. Gene. 2018 Aug 15;667:45-55. doi: 10.1016/j.gene.2018.05.015. Epub 2018 May 9. [Application: Postnatal] Learn More
- Pinto D et al. Comprehensive assessment of array-based platforms and calling algorithms for detection of copy number variants. Nat Biotechnol. 2011 May 8;29(6):512-20. doi: 10.1038/nbt.1852 [Application: General] Learn More
- Alesi V et al. Easychip 8x15k: A New Tool for Detecting Chromosome Anomalies in Low Risk Pregnancies, Supporting and Integrating Standard Karyotype J Genet Syndr Gene Ther 7: 277. doi:10.4172/2157-7412.1000277 [Application: Prenatal] Learn More
- Chau MHK et al. Characteristics and mode of inheritance of pathogenic copy number variants in prenatal diagnosis. Am J Obstet Gynecol. 2019 Nov;221(5):493.e1-493.e11. doi: 10.1016/j.ajog.2019.06.007. Epub 2019 Jun 14. [Application: Prenatal] Learn More
- Ribeiro et al. Upper aerodigestive tract carcinoma: Development of a (epi)genomic predictive model for recurrence and metastasis. Oncol Lett. 2020 May;19(5):3459-3468. doi: 10.3892/ol.2020.11459. Epub 2020 Mar 12 [Application: Cancer] Learn More
- Kaur et al. Clinical impact of chromothriptic complex chromosomal rearrangements in newly diagnosed multiple myeloma. Leuk Res. 2019 Jan;76:58-64. doi: 10.1016/j.leukres.2018.12.005 [Application: Cancer] Learn More
