Agilent Seahorse XF T Cell Metabolic Profiling kit reagents
Seahorse XF T Cell Metabolic Profiling Kit
Each assay kit contains sufficient materials for six full-plate tests and is available in two formats. The XF kit is used with the Agilent Seahorse XF Pro and Agilent XFe96 analyzers. The XFp kit is used with the Agilent Seahorse HS Mini and Agilent XFp analyzers.
- XF Assay Kits, Reagents & Cell Assay Media
Product Details
- Generates robust bioenergetic data linked to critical T cell attributes for antitumor properties: T cell persistence and T cell metabolic fitness
- Provides a comprehensive view of T cell and NK cell metabolism, including quantification of both glycolytic and mitochondrial effects
- Evaluates different construct designs, engineering strategies, starting material selection, or manufacturing conditions
- Includes improved reagents for more accurate results and minimal optimization of uncouplers for different T and NK cell populations
- Reconstitute compounds directly in the assay medium, eliminating the need for DMSO
- Is validated for best performance with the Agilent Seahorse XF Pro analyzer, Agilent XF HS Mini analyzer, Agilent Seahorse XF RPMI medium (pH 7.4), and Agilent Seahorse XF supplements
- Supplies easy-to-interpret data analysis provided by Agilent Seahorse Analytics, a cloud-based platform, simplifying data processing
Automatic measurement of energy metabolism in real time
Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. Seahorse XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time.
Learn More- Application Notes
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Improved CAR-NK Metabolic Fitness Leads to More Persistent Killing and is Predictive of Superior Clinical Outcomes
Research highlight confirming the critical role of metabolic fitness in CAR-NK cells and its importance to achieve superior clinical outcomes in immunotherapy.
- Application Notes
- English
- 25 Sep 2024
- 312.60 KB
NovoCyte Flow Cytometer & Immune Suspension Cells Normalization in Seahorse XF Assays
NovoCyte flow cytometer for immune suspension cell normalization in Seahorse XF assays and assessment of bioenergetic metabolic profile of different cell populations.
- Application Notes
- English
- 31 Oct 2023
- 2.43 MB
Assessing T Cell Bioenergetic Poise and SRC Using Extracellular Flux Analysis
Learn about Agilent Seahorse XF T Cell Metabolic Profiling Kit that provides powerful metabolic measurements linked to T cell persistence and metabolic fitness.
- Application Notes
- English
- 23 Mar 2022
- 1.09 MB
Assessing NK Cell Bioenergetic Metabolism Using Extracellular Flux Analysis
The Agilent Seahorse XF NK cell metabolic profiling assay provides simultaneous measurement of glycolytic and mitochondrial activity. This application note outlines the advantages of the assay and demonstrates its utility in optimizing cell therapy products.
- Application Notes
- English
- 10 Apr 2024
- 3.52 MB
- Case Studies
- Flyers
- Posters
- Software
- Instruction Sheets
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Agilent Seahorse XF T Cell Metabolic Profiling Kit Quick Starter Guide
Agilent Seahorse XF T Cell Metabolic Profiling Kit Quick Starter Guide for the Agilent Seahorse XF Pro analyer and XFe96 analyzer.
- Instruction Sheets
- English
- 11 Apr 2024
- 301.33 KB
Agilent Seahorse XFp T Cell Metabolic Profiling Kit Quick Starter Guide (XFp miniplates)
This quick strater guide outlines the key steps in perfroming assays using the Agilent Seahorse XFp T Cell Metabolic Profiling Kit (PN103771-100) and XFp PDL miniplates with the Agilent Seahorse XF HS Mini analyzer or XFp analyzer.
- Instruction Sheets
- English
- 10 Apr 2024
- 235.17 KB
Agilent Seahorse XFp T Cell Metabolic Profiling Kit Quick Starter Guide (HS miniplates)
This quick strater guide outlines the key steps in perfroming assays using the Agilent Seahorse XFp T Cell Metabolic Profiling Kit (PN103771-100) and XF HS PDL miniplates with the Agilent Seahorse XF HS Mini analyzer.
- Instruction Sheets
- English
- 10 Apr 2024
- 236.90 KB
- Material Safety Data Sheets
- User Manuals
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The Agilent Seahorse XF Pro platform
Transforming Bioenergetics - Again. Featuring new pharma-oriented workflow solutions with improved precision, along with advanced experimental design and analysis tools, you can optimize your workflow from start to finish.
- 17 Jan 2022