Seahorse XF Pro Analyzer Scientists’ Choice Awards 2023 Winner
Seahorse XF Pro Analyzer
Agilent Seahorse XF Pro analyzer measures and reports the oxygen consumption rate (OCR), proton efflux rate (PER) or extracellular acidification rate (ECAR), as well as ATP production rates of live cells in a 96-well format. This analyzer features excellent OCR precision at low rates, verified instrument performance, optimized temperature control, and is automation enabled.
OCR, PER, and ATP production rates are critical measurements of energy metabolism and vital indicators of mitochondrial health, toxicity, glycolysis, and overall cellular (dys)function. Together, these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.
The XF Pro analyzer is also equipped with advanced software, standardized workflows, and advanced data analytics available in the Agilent Seahorse Analytics software. These features greatly simplify the entire XF assay experience, from assay design to data QC and interpretation.
OCR, PER, and ATP production rates are critical measurements of energy metabolism and vital indicators of mitochondrial health, toxicity, glycolysis, and overall cellular (dys)function. Together, these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.
The XF Pro analyzer is also equipped with advanced software, standardized workflows, and advanced data analytics available in the Agilent Seahorse Analytics software. These features greatly simplify the entire XF assay experience, from assay design to data QC and interpretation.
Product Details
Features
- Live cell, real-time analysis of cellular energy metabolism platform in 96-well format
- Multiple parameters reported from each assay well, including OCR, PER or ECAR, and ATP product rates
- Excellent OCR precision at low rates, providing confidence when working with quiescent cell types, such as naïve T cells
- High-sensitivity analysis of as few as 5,000 cells per well using the custom 96-well plate
- Compatible with XF Pro M plate to reduce edge effect
- Four-port injection system with automated mixing feature for assessing immediate cellular responses to substrates, inhibitors, and other compounds in real time
- Verified instrument performance to ensure intraplate and interplate data consistency
- Wide operation environment temperature of 4 to 30 °C, allowing the analyzer to maintain internal assay temperature of 16 to 42 °C
- Advanced Pro Controller software for easy assay protocol creation with user guided interface
- Data Quality View in Wave Pro software for automated flagging of outliers
How It Works
Applications