xCELLigence RTCA SP - Single Plate
The instrument operates in a standard CO2 cell culture incubator and the control unit is housed outside the incubator. Simply plate cells and begin monitoring cell behavior to obtain real-time kinetic data for assay windows that stretch from seconds to days. Online data acquisition and offline data analysis are easy to perform using RTCA Software Pro, which also supports FDA 21 CFR Part 11 compliance.
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Product Details
- xCELLigence RTCA SP model can run one 96-well plate
- Measure cell health, viability, or response to treatments with high sensitivity, accuracy, and reproducibility
- Obtain continuous data at high temporal resolution (from seconds to days), with non-invasive and label-free real-time impedance technology
- Easy workflow allows users to simply add cells to E-plates and begin kinetic measurements at physiological conditions, with minimal hands-on time
- Intuitive RTCA Software Pro allows for analysis of real-time data to generate diverse plot types and automatically calculate parameters such as % of cytolysis, IC50 or KT50
- RTCA Software Pro supports FDA 21 CFR Part 11 compliance to ensure electronic data integrity
Cellular Impedance Explained
The functional unit of a cellular impedance assay is a set of gold microelectrodes fused to the bottom surface of a microtiter plate well. When submersed in an electrically conductive solution (such as buffer or standard tissue culture medium), the application of an electric potential across these electrodes causes electrons to exit the negative terminal, pass through bulk solution, and then deposit onto the positive terminal to complete the circuit.
Learn More- Application Notes
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xCELLigence Portfolio Cancer Immunotherapy Handbook
Recently updated Cancer Immunotherapy Handbook featuring the latest high impact xCELLigence publications and newly released supporting information.
- Application Notes
- English
- 10 Mar 2023
- 10.21 MB
Evaluating Functional Potency of Immunotherapies Targeting Tumors of B Cell Origin
Describes use of a tethering approach to immobilize liquid tumor cell lines on to the surface of gold biosensors embedded in an Agilent E-Plate.
- Application Notes
- English
- 03 Jun 2022
- 1.10 MB
From Classical to Online Monitoring of G-Protein-Coupled Receptor Stimulation in Living Cells
To determine if the stimulation of endogenously expressed GPCRs can be monitored by the xCELLigence system, cells were stimulated with various receptor agonists, triggering either Gs-, Gi-, or Gq-mediated downstream signals.
- Application Notes
- English
- 12 Feb 2021
- 3.07 MB
A New Way to Monitor Virus-Mediated Cytopathogenicity
This application note describes the experimental setup for assessing vesicular stomatitis virus-mediated cytotoxicity of Vero E6 cells and HEK 293 cells using an xCELLigence instrument.
- Application Notes
- English
- 12 Feb 2021
- 358.59 KB
Label-Free Assay for NK Cell‑Mediated Cytolysis
In this application note, a series of experiments demonstrate how the xCELLigence RTCA system can be applied to quantitatively monitor NK cell‑mediated cytolysis without labeling the target cells.
- Application Notes
- English
- 12 Feb 2021
- 699.12 KB
Dynamic Monitoring of G-Protein‑Coupled Receptor Activation in Living Cells
In this application note, a series of experiments demonstrates how the xCELLigence system may be used to monitor functional activation of GPCRs in living cells.
- Application Notes
- English
- 12 Feb 2021
- 1.07 MB
Dynamic Monitoring of Cell Adhesion and Spreading
In this application note, a series of experiments is described to demonstrate that the xCELLigence system is suitable for monitoring cell adhesion and spreading.
- Application Notes
- English
- 12 Feb 2021
- 1007.33 KB
Vaccine and Virology Applications
A collection of studies showing the benefits of xCELLigence RTCA assays over traditional assays. Antiviral drugs, virucides, neutralizing antibodies and other applications are examined.
- Application Notes
- English
- 02 Jul 2020
- 4.37 MB
Infectious Disease Applications
A compilation of studies demonstrating the application of xCELLigence RTCA to the analysis of bacterial toxins, biofilms, virus titer determination, and other areas of disease.
- Application Notes
- English
- 02 Jul 2020
- 4.93 MB
Real-Time Quality Control and Functional Assessment of Mesenchymal Stem Cells for Cellular Therapies
An xCELLigence RTCA was used to develop a label-free assay to functionally assess BMSCs.
- Application Notes
- English
- 02 Jul 2020
- 773.17 KB
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Dell Ownership Transfer Process
Dell Ownership transferring process
- Instruction Sheets
- English
- 24 Aug 2020
- 417.41 KB
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