PLRP-S HPLC Columns new sustainable packaging
PLRP-S for Biomolecules HPLC Columns
The inherently hydrophobic chemistry of the polystyrene/divinylbenzene (PS/DVB) particles means there is no bonded phase required for reversed-phase separations, which makes it a highly reproducible material that is also free from silanols and heavy metal ions. Within the extensive product range are columns suitable for analytical separations, and preparative purifications. Their thermal and chemical stability makes them ideal for purifications that require extreme conditions for sample preparation, compound elution, and column regeneration.
With a wide choice of pore sizes and extended range of operating conditions, PLRP-S provides more options to achieve the optimum separations. Particle sizes range from 3 μm to 50 μm for scale-up from the μg/mg discovery stage to multi-kg cGMP production. Excellent chemical stability, up to 1 M NaOH, permits sanitation and regeneration that increase column lifetime. PLRP-S media batch sizes of up to 600 L are available, providing single batch packing of multiple columns.
- Intact & Subunit Analysis
Product Details
- Contain durable and resilient polymer particles that deliver reproducible results over longer lifetimes
- Thermal and chemical stability for separations, optimization, sanitation, and regeneration increases selectivity and column lifetime
- Comply with USP L21 designation
- Particle sizes range from 3 μm to 50 μm
- Pore sizes (100Å-4000Å) for separations of small molecules to large complexes, proteins, peptides, and polynucleotides
Bio HPLC Columns eSelector Tools
Intuitive process to help you select the right chromatography column for your biopharmaceutical applications.
Preparative HPLC Scaling Calculator
Scale between analytical and prep methods; calculate column capacity, # of injections, run time & mobile phase use.
Oligonucleotide Virtual Lab
Explore proven methods and resources to help advance analysis for the broad range of oligonucleotide workflows.
Videos
- Application Notes
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An In-Depth Analysis of Semaglutide, a Glucagon-Like Peptide-1 Receptor Agonist
In this application note, we explore some of the key parameters when comparing different column chemistries using ion-pair reversed-phase (IP-RP) separation of Semaglutide.
- Application Notes
- English
- 10 May 2024
- 529.96 KB
Agilent Biocolumns Application Compendium - Intact and Subunit Purity
Application compendium for intact and subunit purity
- Application Notes
- English
- 28 Jun 2021
- 3.08 MB
Precise Characterization of Intact mAbs by the Agilent 6545XT AdvanceBio LC/Q-TOF
This app note presents a complete high-throughput workflow solution to analyze a variety of mAb products.
- Application Notes
- English
- 19 Nov 2018
- 1.31 MB
An Integrated Workflow for Intact and Subunits of Monoclonal Antibody Accurate Mass Measurements
An Integrated Workflow for Intact and Subunits of Monoclonal Antibody Accurate Mass Measurements
- Application Notes
- English
- 29 Mar 2018
- 13.65 MB
Measuring Drug-to-Antibody Ratio (DAR) for Antibody-Drug Conjugates(ADCs) with UHPLC/Q-TOF
In this paper, we are investigating an antibody drug conjugate (ADC) with a cysteine linker.
- Application Notes
- English
- 13 Oct 2016
- 1.15 MB
PLRP-S Polymeric Reverse Phase Column for LC/MS Separation of mAbs and ADC
Agilent PLRP-S column demonstrates superior separation performance for the analysis of mAbs and ADC at both intact and fragment levels
- Application Notes
- English
- 01 Aug 2016
- 287.58 KB
Improved Column Lifetime with Thermally Stable Polymer Columns for Oligonucleotide Ion-Pair RP HPLC
Evaluation of the thermal stability and lifetime of the columns subjected to 100 cycles of 25-minute long gradient cycles at either 80 °C (PLRP-S) or 60 °C (all other columns)
- Application Notes
- English
- 27 Mar 2013
- 1003.98 KB
High performance DNA oligonucleotide purification using Agilent TOP-DNA
Because of the significant progress achieved in the solid phase chemical synthesis of oligonucleotides during the past four decades, synthetic oligonucleotides...
- Application Notes
- English
- 26 Sep 2012
- 707.71 KB
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Bio LC Column User Guides
User guides contain helpful information to ensure the best performance from your column, such as operating parameters, cleaning and storage guidelines, usage tips, and suggested starting conditions.
- User Manuals
Agilent Wins Multiple 2022 Scientists' Choice Awards
Agilent received two 2022 Scientists’ Choice Awards for Drug Discovery & Development, Virtual Event of the Year and Innovative Content of the Year, at the SLAS2022 International Conference & Exhibition on February 7th, 2022. The Scientists’ Choice Awards recognize the world’s most innovative and effective analytical science technologies and communications.
- Agilent Wins Multiple 2022 Scientists' Choice Awards
- 15 Feb 2022